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  • KD/KO Validated

FMNL1 Polyclonal antibody

FMNL1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

Human and More (2)

Applications

WB, IP, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 27834-1-AP

Synonyms

C17orf1, C17orf1B, CLL associated antigen KW 13, FHOD4, FMNL, FMNL1, formin like 1, Formin like protein 1, KW 13, Leukocyte formin



Tested Applications

Positive WB detected inRaji cells, Jurkat cells, HeLa cells
Positive IP detected inCaki-1
Positive IHC detected inhuman lymphoma tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inU2OS cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27834-1-AP targets FMNL1 in WB, IP, IHC, IF, ELISA applications and shows reactivity with Human samples.

Tested Reactivity Human
Cited Reactivityhuman, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FMNL1 fusion protein Ag27312
Full Name formin-like 1
Observed Molecular Weight 125-150 kDa
GenBank Accession NumberBC111564
Gene Symbol FMNL1
Gene ID (NCBI) 752
RRIDAB_2880987
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FMNL1,also named as C17orf1, C17orf1B and FMNL, belongs to the formin homology family. It may play a role in the control of cell motility and survival of macrophages. FNMLl protein may be involved in anti-apoptosis mechanisms. It is overexpressed in lymphoid malignances and is found associated with Akt. 27834-1-AP is a rabbit polyclonal antibody recognises the 125-150 kDa protein.(PMID:16494944)

Protocols

Product Specific Protocols
WB protocol for FMNL1 antibody 27834-1-APDownload protocol
IHC protocol for FMNL1 antibody 27834-1-APDownload protocol
IF protocol for FMNL1 antibody 27834-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Theranostics

Stiff matrix induced srGAP2 tension gradients control migration direction in triple-negative breast cancer

Authors - Chen Li
  • KD Validated
humanIHC

Gastric Cancer

FHOD1 and FMNL1 formin proteins in intestinal gastric cancer: correlation with tumor-infiltrating T lymphocytes and molecular subtypes.

Authors - Naziha Mansuri
humanWB

Cell Rep

Substrate rigidity dictates colorectal tumorigenic cell stemness and metastasis via CRAD-dependent mechanotransduction.

Authors - Yuhan Chang
humanIHC

Biomed Res Int

Systematic Characterization of Expression Patterns and Immunocorrelations of Formin-Like Genes in Breast Cancer

Authors - Erli Gao
mouseIHC

Clin Immunol

Formin protein DIAPH1 positively regulates PD-L1 expression and predicts the therapeutic response to anti-PD-1/PD-L1 immunotherapy

Authors - Jie Mei
humanIF

J Cancer

Formin protein FMNL1 is a biomarker for tumor-infiltrating immune cells and associated with well immunotherapeutic response

Authors - Guomin Lu