FMNL1 Polyclonal antibody

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• FMNL1 Polyclonal antibody (27834-1-AP)

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• KD/KO Validated

FMNL1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 27834-1-AP Copied!

Publications (8)

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Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

CLL-associated antigen KW-13, FMNL, Formin-like protein 1

• 

  WB analysis using 27834-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27834-1-AP (FMNL1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of HeLa using 27834-1-AP

  WB result of FMNL1 antibody (27834-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FMNL1 transfected HeLa cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of Jurkat using 27834-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 27834-1-AP (FMNL1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of Caki-1 using 27834-1-AP

  IP result of anti-FMNL1 (IP:27834-1-AP, 2ug; Detection:27834-1-AP 1:2000) with Caki-1 cells lysate 1000 ug. The result was provided by Keli Chai, Xuzhou Medical University.

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  IHC staining of human lymphoma using 27834-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27834-1-AP (FMNL1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lymphoma using 27834-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 27834-1-AP (FMNL1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of U2OS using 27834-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U2OS cells using 27834-1-AP (FMNL1 antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: Raji cells, HeLa cells, Jurkat cells
• Positive IP detected in: Caki-1
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: U2OS cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27834-1-AP targets FMNL1 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag27312

  Product name: Recombinant human FMNL1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 357-501 aa of BC111564

  Sequence: LYLERLRLTESDKLQVQIQAYLDNIFDVGALLEDTETKNAVLEHMEELQEQVALLTERLRDAENESMAKIAELEKQLSQARKELETLRERFSESTAMGPSRRPPEPEKAPPAAPTRPSALELKVEELEEKGLIRILRGPGDAVSI

• Full Name: formin-like 1
• Observed Molecular Weight: 125-150 kDa
• GenBank Accession Number: BC111564
• Gene Symbol: FMNL1
• Gene ID (NCBI): 752
• RRID: AB_2880987
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O95466
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FMNL1,also named as C17orf1, C17orf1B and FMNL, belongs to the formin homology family. It may play a role in the control of cell motility and survival of macrophages. FNMLl protein may be involved in anti-apoptosis mechanisms. It is overexpressed in lymphoid malignances and is found associated with Akt. 27834-1-AP is a rabbit polyclonal antibody recognises the 125-150 kDa protein.(PMID:16494944)

Publications

What published studies show

The FMNL1 Polyclonal antibody (Cat# 27834-1-AP) has 8 citations. They appear in Theranostics, Developmental Cell, Cell Reports, Gastric Cancer, Clinical Immunology, Journal of Cancer, Biomedical Research International, and Journal of International Medical Research. Research fields span cancer biology (breast, colorectal, gastric, and pancreatic cancer), mechanotransduction, formin protein function, and tumor immunotherapy/immune response.

Automatically generated from the 8 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Theranostics; Stiff matrix induced srGAP2 tension gradients control migration direction in triple-negative breast cancer; Authors - Chen Li ; KD Validated
human	WB	Dev Cell; Vimentin intermediate filaments coordinate actin stress fibers and podosomes to determine the extracellular matrix degradation by macrophages; Authors - Xinyi Huang
human	WB	Cell Rep; Substrate rigidity dictates colorectal tumorigenic cell stemness and metastasis via CRAD-dependent mechanotransduction.; Authors - Yuhan Chang
human	IHC	Gastric Cancer; FHOD1 and FMNL1 formin proteins in intestinal gastric cancer: correlation with tumor-infiltrating T lymphocytes and molecular subtypes.; Authors - Naziha Mansuri
mouse	IHC	Clin Immunol; Formin protein DIAPH1 positively regulates PD-L1 expression and predicts the therapeutic response to anti-PD-1/PD-L1 immunotherapy; Authors - Jie Mei
human	IF	J Cancer; Formin protein FMNL1 is a biomarker for tumor-infiltrating immune cells and associated with well immunotherapeutic response; Authors - Guomin Lu