FMO2 Polyclonal antibody

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• FMO2 Polyclonal antibody (15725-1-AP)

FMO2 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 15725-1-AP Copied!

Publications (1)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Synonyms

Dimethylaniline oxidase 2, FMO 1B1, FMO 2, FMO1B1, FMO2

• 

  WB analysis using 15725-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15725-1-AP (FMO2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 15725-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15725-1-AP (FMO2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human lung cancer using 15725-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15725-1-AP (FMO2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse lung tissue
• Positive IHC detected in: human lung cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15725-1-AP targets FMO2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8464

  Product name: Recombinant human FMO2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 27-386 aa of BC005894

  Sequence: EPTCFERTEDIGGVWRFKENVEDGRASIYQSVVTNTSKEMSCFSDFPMPEDFPNFLHNSKLLEYFRIFAKKFDLLKYIQFQTTVLSVRKCPDFSSSGQWKVVTQSNGKEQSAVFDAVMVCSGHHILPHIPLKSFPGMERFKGQYFHSRQYKHPDGFEGKRILVIGMGNSGSDIAVELSKNAAQVFISTRHGTWVMSRISEDGYPWDSVFHTRFRSMLRNVLPRTAVKWMIEQQMNRWFNHENYGLEPQNKYIMKEPVLNDDVPSRLLCGAIKVKSTVKELTETSAIFEDGTVEENIDVIIFATGYSFSFPFLEDSLVKVENNMVSLYKYIFPAHLDKSTLACIGLIQPLGSIFPTAELQA

• Full Name: flavin containing monooxygenase 2
• Calculated Molecular Weight: 471aa,54 kDa; 534aa,61 kDa
• Observed Molecular Weight: 50-60 kDa
• GenBank Accession Number: BC005894
• Gene Symbol: FMO2
• Gene ID (NCBI): 2327
• RRID: AB_2278518
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q99518
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FMO2, belongs to FMO (flavin containing monooxygenase) family, which catalyzes the oxidation of soft nucleophilic heteroatom centers in drugs, pesticides, and xenobiotics. FMO family has 1-5 members, with homologous sequences. This antibody is specially against FMO2. It does not bind other FMOs.

Publications

What published studies show

The FMO2 Polyclonal antibody (Cat# 15725-1-AP) has 1 citation. It was published in Korean Journal of Physiology and Pharmacology (2025), in a study examining age-related changes in diurnal expression of inflammatory mediators in the brain and peripheral blood of mice. The research fields associated with this citation include aging, circadian biology, neuroinflammation, and immunology.

Automatically generated from the 1 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
	WB	Korean J Physiol Pharmacol; Age-related changes in diurnal expression of inflammatory mediators in the brain and peripheral blood of male and female mice; Authors - Eun-Mi Lee