Tested Applications
| Positive WB detected in | mouse lung tissue, A549 cells, pig lung tissue |
| Positive IHC detected in | human lung cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunohistochemistry (IHC) | IHC : 1:150-1:600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:400-1:1600 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
67019-1-Ig targets FMO2 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, pig samples.
| Tested Reactivity | human, mouse, pig |
| Cited Reactivity | human, mouse |
| Host / Isotype | Mouse / IgG1 |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag8610 Product name: Recombinant human FMO2 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 27-386 aa of BC005894 Sequence: EPTCFERTEDIGGVWRFKENVEDGRASIYQSVVTNTSKEMSCFSDFPMPEDFPNFLHNSKLLEYFRIFAKKFDLLKYIQFQTTVLSVRKCPDFSSSGQWKVVTQSNGKEQSAVFDAVMVCSGHHILPHIPLKSFPGMERFKGQYFHSRQYKHPDGFEGKRILVIGMGNSGSDIAVELSKNAAQVFISTRHGTWVMSRISEDGYPWDSVFHTRFRSMLRNVLPRTAVKWMIEQQMNRWFNHENYGLEPQNKYIMKEPVLNDDVPSRLLCGAIKVKSTVKELTETSAIFEDGTVEENIDVIIFATGYSFSFPFLEDSLVKVENNMVSLYKYIFPAHLDKSTLACIGLIQPLGSIFPTAELQA Predict reactive species |
| Full Name | flavin containing monooxygenase 2 |
| Calculated Molecular Weight | 471aa,54 kDa; 534aa,61 kDa |
| Observed Molecular Weight | 50-60 kDa |
| GenBank Accession Number | BC005894 |
| Gene Symbol | FMO2 |
| Gene ID (NCBI) | 2327 |
| RRID | AB_2882335 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | Q99518 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FMO2 antibody 67019-1-Ig | Download protocol |
| IHC protocol for FMO2 antibody 67019-1-Ig | Download protocol |
| WB protocol for FMO2 antibody 67019-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FMO2 Monoclonal antibody (3F1F7), catalog number 67019-1-Ig, has 5 total citations published in Cell Metabolism, Science Advances, Cells, Stem Cells Translational Medicine, and Frontiers in Cell and Developmental Biology. Research fields span reproductive immunology (decidualization and recurrent spontaneous abortion), cardiology (mitochondrial transfer in sepsis), stem cell biology (limbal progenitor differentiation), extracellular vesicle-mediated photoaging protection, and flavin-containing monooxygenase metabolism and stress resistance.
| Species | Application | Title |
|---|---|---|
Cell Metab Endometrial stromal cell-derived TMAO sustains decidualization to prevent recurrent spontaneous abortion. | ||
Sci Adv Cytoskeletal remodeling promotes tunneling nanotube formation and drives cardiac resident cell mitochondrial transfer in sepsis. | ||
Cells Differentiation Trajectory of Limbal Stem and Progenitor Cells under Normal Homeostasis and upon Corneal Wounding. | ||
Stem Cells Transl Med Mesenchymal stromal cells-derived small extracellular vesicles protect against UV-induced photoaging via regulating pregnancy zone protein | ||
Front Cell Dev Biol Flavin-Containing Monooxygenases Are Conserved Regulators of Stress Resistance and Metabolism. |
