FMO4 Polyclonal antibody

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  WB analysis of HEK-293 using 15394-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15394-1-AP (FMO4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

FMO4 Polyclonal Antibody for WB, ELISA

Cat No. 15394-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

Dimethylaniline oxidase 4, FMO 4, FMO2, FMO4

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Tested Applications

• Positive WB detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15394-1-AP targets FMO4 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FMO4 fusion protein Ag7590
• Full Name: flavin containing monooxygenase 4
• Calculated Molecular Weight: 63 kDa
• Observed Molecular Weight: 63 kDa
• GenBank Accession Number: BC002780
• Gene Symbol: FMO4
• Gene ID (NCBI): 2329
• RRID: AB_2105402
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P31512
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FMO4, belongs to FMO (flavin containing monooxygenase) family, which catalyzes the oxidation of soft nucleophilic heteroatom centers in drugs, pesticides, and xenobiotics. FMO family has 1-5 members. 2 splicing variants of FMO4 were reported, according to 56kda and 63kda bands in SDS page. This antibody, generated by N-330 amino-acids of FMO4, can recognize the 2 variants and also cross-react with other FMO proteins.