FMO5 Polyclonal antibody

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FMO5 Polyclonal Antibody for WB, IP, ELISA

Cat No. 13699-1-AP Copied!

Publications (6)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IP, ELISA

Synonyms

Baeyer-Villiger monooxygenase 1, Dimethylaniline monooxygenase [N-oxide-forming] 5, EC:1.14.13.-, EC:1.14.13.8, EC:1.6.3.1

• 

  WB analysis of human liver using 13699-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 13699-1-AP (FMO5 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse lung using 13699-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13699-1-AP (FMO5 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of mouse lung using 13699-1-AP

  IP result of anti-FMO5 (IP:13699-1-AP, 4ug; Detection:13699-1-AP 1:500) with mouse lung tissue lysate 4000ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: human liver tissue, mouse lung tissue
• Positive IP detected in: mouse lung tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

13699-1-AP targets FMO5 in WB, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag4604

  Product name: Recombinant human FMO5 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 34-285 aa of BC035687

  Sequence: RTDDIGGLWRFQENPEEGRASIYKSVIINTSKEMMCFSDYPIPDHYPNFMHNAQVLEYFRMYAKEFDLLKYIRFKTTVCSVKKQPDFATSGQWEVVTESEGKKEMNVFDGVMVCTGHHTNAHLPLESFPGIEKFKGQYFHSRDYKNPEGFTGKRVIIIGIGNSGGDLAVEISQTAKQVFLSTRRGAWILNRVGDYGYPADVLFSSRLTHFIWKICGQSLANKYLEKKINQRFDHEMFGLKPKHRSKDIALTE

• Full Name: flavin containing monooxygenase 5
• Calculated Molecular Weight: 533 aa, 60 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC035687
• Gene Symbol: FMO5
• Gene ID (NCBI): 2330
• RRID: AB_2105409
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P49326
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Microsomal flavin-containing monooxygenases (FMOs) [dimethylaniline monooxygenase (N-oxide forming) catalyze the FAD-, NADPH- and O2-dependent oxidation of a large number of structurally diverse compounds, including drugs, pesticides, and industrial chemicals containing a soft nucleophile(PMID:12488558 ). FMO5, which belongs to the FMO family, is a lesser component of human liver microsomes and is present at about one-third the level of FMO3. FMO5 protein is also present at very low levels in kidney, however, FMO5 exhibits a severely restricted substrate specificity for most drugs and other xenobiotics examined to date(PMID:10950857). It has 2 isoforms produced by alternative splicing.

Publications

What published studies show

Anti-FMO5 antibody (Cat# 13699-1-AP) has 6 citations published in Cell Metabolism, Materials Today Bio, Biochemical Pharmacology, Frontiers in Pharmacology, Frontiers in Cell and Developmental Biology, and Drug Metabolism and Disposition. Research fields span reproductive biology (decidualization), bone homeostasis, metabolic ageing, non-alcoholic fatty liver disease, stress resistance, and circadian rhythm regulation of drug metabolism.

Automatically generated from the 6 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Metab; Endometrial stromal cell-derived TMAO sustains decidualization to prevent recurrent spontaneous abortion.; Authors - Yu-Ling Chen
		Mater Today Bio; Ceria nanoparticles guard against glucocorticoid induced bone homeostasis imbalance through the promotion of osteogenesis and inhibition of osteoclastogenesis; Authors - Anwei Zhang
mouse	WB	Biochem Pharmacol; The phenotype of a knockout mouse identifies flavin-containing monooxygenase 5 (FMO5) as a regulator of metabolic ageing.; Authors - Sandra G Gonzalez Malagon ; KO Validated
mouse	WB	Front Pharmacol; Puerarin ameliorates non-alcoholic fatty liver disease by inhibiting lipid metabolism through FMO5; Authors - Zhaoyi Li
human	WB	Front Cell Dev Biol; Flavin-Containing Monooxygenases Are Conserved Regulators of Stress Resistance and Metabolism.; Authors - Shijiao Huang
mouse	WB	Drug Metab Dispos; The Molecular Mechanism Regulating Diurnal Rhythm of Flavin-Containing Monooxygenase 5 in Mouse Liver.; Authors - Min Chen