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Fibromodulin Polyclonal antibody

Fibromodulin Polyclonal Antibody for WB, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Conjugate

Unconjugated

Cat no : 13281-1-AP

Synonyms

FMOD, SLRR2E, KSPG fibromodulin, Keratan sulfate proteoglycan fibromodulin, FM

Validation Data Gallery

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  • Western Blot (WB) analysis of human liver tissue using Fibromodulin Polyclonal antibody (13281-1-AP)

    WB analysis of human liver using 13281-1-AP

    human liver tissue were subjected to SDS PAGE followed by western blot with 13281-1-AP (Fibromodulin antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

  • Immunofluorescence (IF) / fluorescent staining of HeLa cells using Fibromodulin Polyclonal antibody (13281-1-AP)

    IF Staining of HeLa using 13281-1-AP

    Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13281-1-AP (Fibromodulin antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

Tested Applications

Positive WB detected inhuman liver tissue
Positive IF/ICC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunofluorescence (IF)/ICCIF/ICC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

WBSee 1 publications below

Product Information

13281-1-AP targets Fibromodulin in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Fibromodulin fusion protein Ag4030
Full Name fibromodulin
Calculated Molecular Weight 376 aa, 43 kDa
Observed Molecular Weight 43 kDa
GenBank Accession NumberBC035281
Gene Symbol Fibromodulin
Gene ID (NCBI) 2331
RRIDAB_2105531
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fibromodulin (FMOD), an extracellular matrix protein, belongs to the small leucine-rich proteoglycan family. Fibromodulin is a collagen-binding protein present in many types of connective tissues, e.g. cartilage, tendon, skin, sclera and cornea (PMID: 2531085). It is involved in fibrillogenesis, cell adhesion, and cytokine activity modulation (PMID: 15741214). Fibromodulin may participate in the assembly of the extracellular matrix as it interacts with type I and type II collagen fibrils and inhibits fibrillogenesis in vitro. It may also regulate TGF-beta activities by sequestering TGF-beta into the extracellular matrix. The calculated molecular mass of fibromodulin is 43 kDa, extracellular fibromodulin appears as one single band of 59 kDa due to glycosylation (PMID: 2531085; 22138190).

Protocols

Product Specific Protocols
WB protocol for Fibromodulin antibody 13281-1-APDownload protocol
IF protocol for Fibromodulin antibody 13281-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

iScience

Fibromodulin overexpression drives oral squamous cell carcinoma via activating downstream EGFR signaling

Authors - Lingyun Xia
View Article