FN3K Polyclonal antibody

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  WB analysis of HeLa using 14293-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 14293-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14293-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 14293-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 14293-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14293-1-AP (FN3K antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 14293-1-AP

  IP result of anti-FN3K (IP:14293-1-AP, 3ug; Detection:14293-1-AP 1:500) with HeLa cells lysate 1840ug.

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  IHC staining of human kidney using 14293-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14293-1-AP (FN3K antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14293-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14293-1-AP (FN3K antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 14293-1-AP

  Immunofluorescent analysis of HeLa cells using 14293-1-AP (FN3K antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

FN3K Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Cat No. 14293-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, IP, ELISA

Synonyms

Protein-ribulosamine 3-kinase FN3K, Protein-psicosamine 3-kinase FN3K, Fructosamine-3-kinase, fructosamine 3 kinase, EC:2.7.1.172

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Tested Applications

• Positive WB detected in: HeLa cells, human brain tissue, human heart tissue, human kidney tissue, mouse skeletal muscle tissue
• Positive IP detected in: HeLa cells
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below

Product Information

14293-1-AP targets FN3K in WB, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag5617

  Product name: Recombinant human FN3K protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-309 aa of BC042680

  Sequence: MEQLLRAELRTATLRAFGGPGAGCISEGRAYDTDAGPVFVKVNRRTQARQMFEGEVASLEALRSTGLVRVPRPMKVIDLPGGGAAFVMEHLKMKSLSSQASKLGEQMADLHLYNQKLREKLKEEENTVGRRGEGAEPQYVDKFGFHTVTCCGFIPQVNEWQDDWPTFFARHRLQAQLDLIEKDYADREARELWSRLQVKIPDLFCGLEIVPALLHGDLWSGNVAEDDVGPIIYDPASFYGHSEFELAIALMFGGFPRSFFTAYHRKIPKAPGFDQRLLLYQLFNYLNHWNHFGREYRSPSLGTMRRLLK

• Full Name: fructosamine 3 kinase
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 35 kDa
• GenBank Accession Number: BC042680
• Gene Symbol: FN3K
• Gene ID (NCBI): 64122
• RRID: AB_2105975
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H479
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	iScience; Amiloride reduces fructosamine-3-kinase expression to restore sunitinib sensitivity in renal cell carcinoma; Authors - Yuanyuan Bai
human	WB	Cell Rep; A microscopy-based screen identifies cellular kinases modulating mitochondrial translation; Authors - Roya Yousefi