Toca 1 Polyclonal antibody

Toca 1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Cat no : 55055-1-AP

Synonyms

C1orf39, FNBP1L, formin binding protein 1 like, Toca 1, TOCA1

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Validation Data Gallery

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  WB analysis of mouse brain using 55055-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55055-1-AP (Toca 1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: mouse brain tissue

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55055-1-AP targets Toca 1 in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: formin binding protein 1-like
• Calculated Molecular Weight: 70 kDa
• Observed Molecular Weight: 63-75 kDa
• GenBank Accession Number: NM_017737
• Gene Symbol: Toca 1
• Gene ID (NCBI): 54874
• RRID: AB_10858792
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Toca-1, also named as FNBP1L or C1orf39, belongs to the FNBP1 family. It is required to coordinate membrane tubulation with reorganization of the actin cytoskeleton during endocytosis. Toca-1 may bind to lipids such as phosphatidylinositol 4,5-bisphosphate and phosphatidylserine and promote membrane invagination and the formation of tubules. Also promotes CDC42-induced actin polymerization by activating the WASL/N-WASP-WASPIP/WIP complex, the predominant form of WASL/N-WASP in cells. The antibody is specific to Toca-1.