FNDC3A Polyclonal antibody

• 

  WB analysis of HEK-293 using 25240-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 25240-1-AP (FNDC3A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse placenta using 25240-1-AP

  mouse placenta tissue were subjected to SDS PAGE followed by western blot with 25240-1-AP (FNDC3A Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of U-251 using 25240-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed U-251 cells using FNDC3A antibody (25240-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

FNDC3A Polyclonal Antibody for WB, IF/ICC, ELISA

Cat No. 25240-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IF/ICC, ELISA

Synonyms

FNDC3, FNDC3A, HUGO, KIAA0970

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Tested Applications

• Positive WB detected in: HEK-293 cells, mouse placenta tissue
• Positive IF/ICC detected in: U-251 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25240-1-AP targets FNDC3A in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18736

  Product name: Recombinant human FNDC3A protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 111-250 aa of BC132812

  Sequence: LHRSPHPPLPGFIPVPTMMPPPPRHMYSPVTGAGDMTTQYMPQYQSSQVYGDVDAHSTHGRSNFRDERSSKTYERLQKKLKDRQGTQKDKMSSPPSSPQKCPSPINEHNGLIKGQIAGGINTGSAKIKSGKGKGGTQVDT

• Full Name: fibronectin type III domain containing 3A
• Calculated Molecular Weight: 1198 aa, 132 kDa
• Observed Molecular Weight: 120-130 kDa
• GenBank Accession Number: BC132812
• Gene Symbol: FNDC3A
• Gene ID (NCBI): 22862
• RRID: AB_2879982
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y2H6
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
		Biochim Biophys Acta Mol Cell Res; Silencing of fibronectin type III domain-containing protein 3A (FNDC3A) attenuates epithelial-to-mesenchymal transition (EMT), cancer invasion, and stemness in triple-negative breast cancer (TNBC); Authors - Nanaka Wada