FNDC3B Polyclonal antibody

• Featured Product
• KD/KO Validated

FNDC3B Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 22605-1-AP

Synonyms

Factor for adipocyte differentiation 104, FAD104, fibronectin type III domain containing 3B, FNDC3B, HCV NS5A binding protein 37

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Validation Data Gallery

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  WB analysis of 4T1 using 22605-1-AP

  4T1 cells were subjected to SDS PAGE followed by western blot with 22605-1-AP (FNDC3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of C6 using 22605-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 22605-1-AP (FNDC3B antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 22605-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 22605-1-AP (FNDC3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 22605-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 22605-1-AP (FNDC3B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HepG2 using 22605-1-AP

  IP result of anti-FNDC3B (IP:22605-1-AP, 4ug; Detection:22605-1-AP 1:1000) with HepG2 cells lysate 2400ug.

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  IHC staining of human liver using 22605-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22605-1-AP (FNDC3B Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 22605-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 22605-1-AP (FNDC3B Antibody) at dilution of 1:200 (under 40x lens).

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Tested Applications

• Positive WB detected in: 4T1 cells, HL-60 cells, HepG2 cells, C6 cells
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Product Information

The immunogen of 22605-1-AP is FNDC3B Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FNDC3B fusion protein Ag18357
• Full Name: fibronectin type III domain containing 3B
• Calculated Molecular Weight: 1204 aa, 133 kDa
• Observed Molecular Weight: 150 kDa, 70 kDa
• GenBank Accession Number: BC039297
• Gene Symbol: FNDC3B
• Gene ID (NCBI): 64778
• RRID: AB_2879133
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB,IHC,IF	Cancer Sci; FNDC3B 3'-UTR shortening escapes from microRNA-mediated gene repression and promotes nasopharyngeal carcinoma progression.; Authors - Ying-Qing Li ; KD Validated
human	WB	Pediatr Res; Hirschsprung's disease: key microRNAs and target genes.; Authors - Mei Hong
human	WB	Cancers (Basel); Integrated Analysis of RNA-Binding Proteins in Glioma.; Authors - Zhixing Wang
human	WB	J Cell Physiol; FNDC3B circular RNA promotes the migration and invasion of gastric cancer cells via the regulation of E-cadherin and CD44 expression.; Authors - Yuling Hong
human	WB,IHC	Am J Transl Res; EYA1 promotes cell migration and tumor metastasis in hepatocellular carcinoma.; Authors - Deguang Kong
human	WB	J Cell Commun Signal; CircRNA hsa_circ_0001627 aggravates cervical cancer progression through upregulation of FNDC3B and activating PI3K/mTOR signaling pathway; Authors - Yan Li