FNTA Polyclonal antibody

• Featured Product
• KD/KO Validated

FNTA Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Conjugate

Unconjugated

Publications

2

Cat no : 12274-1-AP

Synonyms

FNTA, FPTA, FTase alpha, GGTase I alpha, PGGT1A, PTAR2

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Validation Data Gallery

• 

  WB analysis using 12274-1-AP

  JAR cells were subjected to SDS PAGE followed by western blot with 12274-1-AP (FNTA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 12274-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 12274-1-AP (FNTA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of 3T3-L1 using 12274-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 12274-1-AP (FNTA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: JAR cells, NIH/3T3 cells, 3T3-L1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 2 publications below

Product Information

12274-1-AP targets FNTA in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FNTA fusion protein Ag2918
• Full Name: farnesyltransferase, CAAX box, alpha
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 44 kDa
• GenBank Accession Number: BC017029
• Gene Symbol: FNTA
• Gene ID (NCBI): 2339
• RRID: AB_2106172
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB	Front Pharmacol; Lonafarnib Inhibits Farnesyltransferase via Suppressing ERK Signaling Pathway to Prevent Osteoclastogenesis in Titanium Particle-Induced Osteolysis.; Authors - Linke Huang ; KD Validated
human	WB	Cell Biol Int; Silencing geranylgeranyltransferase I inhibits the migration and invasion of salivary adenoid cystic carcinoma through RhoA/ROCK1/MLC signaling and suppresses proliferation through cell cycle regulation; Authors - Zengpeng Chi ; KD Validated