FNTB Polyclonal antibody

• Featured Product
• KD/KO Validated

FNTB Polyclonal Antibody for IHC, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC,ELISA

Conjugate

Unconjugated

Cat no : 11507-1-AP

Synonyms

FNTB, FPTB, FTase beta

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Validation Data Gallery

• 

  WB analysis of mouse heart using 11507-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 11507-1-AP (FNTB antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 11507-1-AP

  WB result of FNTB antibody (11507-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FNTB transfected A431 cells.

• 

  WB analysis of mouse skin using 11507-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 11507-1-AP (FNTB Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 11507-1-AP

  IP result of anti-FNTB (IP:11507-1-AP, 4ug; Detection:11507-1-AP 1:1000) with HeLa cells lysate 800ug.

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Tested Applications

• Positive WB detected in: mouse heart tissue, A431 cells, mouse skin tissue
• Positive IP detected in: HeLa cells
• Positive IHC detected in: human lymphoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

11507-1-AP targets FNTB in WB, IP, IHC,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FNTB fusion protein Ag2061
• Full Name: farnesyltransferase, CAAX box, beta
• Calculated Molecular Weight: 437 aa, 49 kDa
• Observed Molecular Weight: 49 kDa, 44 kDa
• GenBank Accession Number: BC020232
• Gene Symbol: FNTB
• Gene ID (NCBI): 2342
• RRID: AB_2106301
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FNTB is the catalytic β subunit of farnesyltransferase which is an alpha/beta heterodimeric enzyme attaching a farnesyl group to a single cysteine in several cellular proteins like RAS and facilitating their translocation from the cytoplasm to the plasma membrane. Farnesyltransferase inhibitors have been developed as ant-tumor agents. FNTB is required for the homeostasis of skin keratinocytes.

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