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  • KD/KO Validated

FOLR1 Polyclonal antibody

FOLR1 Polyclonal Antibody for WB, IF, IHC, ELISA

Host / Isotype

Rabbit / IgG


human and More (3)





Cat no : 23355-1-AP


Adult folate binding protein, FBP, Folate receptor 1, folate receptor 1 (adult), Folate receptor alpha, Folate receptor, adult, FOLR, FOLR1, FR alpha, KB cells FBP, MOv18

Tested Applications

Positive WB detected inHeLa cells, JAR cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

Western Blot (WB)WB : 1:2000-1:12000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

23355-1-AP targets FOLR1 in WB, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, rat, Camelus bactrianus
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FOLR1 fusion protein Ag19959
Full Name folate receptor 1 (adult)
Calculated Molecular Weight 257 aa, 30 kDa
Observed Molecular Weight 38 kDa
GenBank Accession NumberBC002947
Gene Symbol FOLR1
Gene ID (NCBI) 2348
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Folate receptor 1 (FOLR1), also known as folate receptor alpha or adult folate-binding protein (FBP), is a 38-kDa glycoprotein belonging to the folate receptor family (PMID:15094207; 23528302). The receptor binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. FOLR1 is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. FOLR1 expression is often limited to the apical surfaces of epithelium in the lung, kidney and choroid plexus but is differentially overexpressed in a variety of solid tumors such as ovarian cancer, non-small cell lung cancer, breast cancer, kidney cancer and high-grade osteosarcoma (PMID: 23528302; 23357463).


Product Specific Protocols
WB protocol for FOLR1 antibody 23355-1-APDownload protocol
IHC protocol for FOLR1 antibody 23355-1-APDownload protocol
IF protocol for FOLR1 antibody 23355-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



ACS Nano

Synchronization of Nanoparticle Sensitization and Radiosensitizing Chemotherapy through Cell Cycle Arrest Achieving Ultralow X-ray Dose Delivery to Pancreatic Tumors.

Authors - Divinah Manoharan

J Am Chem Soc

Cancer Selective Target Degradation by Folate-Caged PROTACs.

Authors - Jing Liu
  • KD Validated

ACS Appl Mater Interfaces

Design and Fabrication of Multifunctional Sericin Nanoparticles for Tumor Targeting and pH-Responsive Subcellular Delivery of Cancer Chemotherapy Drugs.

Authors - Lei Huang

J Med Chem

Folate-Guided Protein Degradation by Immunomodulatory Imide Drug-Based Molecular Glues and Proteolysis Targeting Chimeras.

Authors - He Chen
  • KD Validated

BMC Cancer

Cancer-associated fibroblasts induce sorafenib resistance of hepatocellular carcinoma cells through CXCL12/FOLR1

Authors - Jiali Zhao


PMID: 28767041

Authors - Yuwei Hu


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


Marvin (Verified Customer) (12-01-2022)

The antibody had non-specific staining of the cytosol of MDA-MB-231 cells while performing immunofluorescent analysis.

  • Applications: Western Blot, Immunofluorescence
  • Primary Antibody Dilution: 1:50
  • Cell Tissue Type: Monolayer of MDA-MB-231 cells
FOLR1 Antibody Western Blot,Immunofluorescence validation (1:50 dilution) in Monolayer of MDA-MB-231 cells (Cat no:23355-1-AP)