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FOLR1 Polyclonal antibody

FOLR1 Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 23355-1-AP

Synonyms

Adult folate binding protein, FBP, Folate receptor 1, folate receptor 1 (adult), Folate receptor alpha, Folate receptor, adult, FOLR, FOLR1, FR alpha, KB cells FBP, MOv18



Tested Applications

Positive WB detected inHeLa cells
Positive IHC detected inhuman breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 23355-1-AP is FOLR1 Fusion Protein expressed in E. coli.

Tested Reactivity human
Cited Reactivity Camelus bactrianus, human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FOLR1 fusion protein Ag19959
Full Name folate receptor 1 (adult)
Calculated molecular weight 257 aa, 30 kDa
Observed molecular weight 38 kDa
GenBank accession numberBC002947
Gene symbol FOLR1
Gene ID (NCBI) 2348
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

Folate receptor 1 (FOLR1), also known as folate receptor alpha or adult folate-binding protein (FBP), is a 38-kDa glycoprotein belonging to the folate receptor family (PMID:15094207; 23528302). The receptor binds to folate and reduced folic acid derivatives and mediates delivery of 5-methyltetrahydrofolate to the interior of cells. FOLR1 is a secreted protein that either anchors to membranes via a glycosyl-phosphatidylinositol linkage or exists in a soluble form. FOLR1 expression is often limited to the apical surfaces of epithelium in the lung, kidney and choroid plexus but is differentially overexpressed in a variety of solid tumors such as ovarian cancer, non-small cell lung cancer, breast cancer, kidney cancer and high-grade osteosarcoma (PMID: 23528302; 23357463).

Protocols

Product Specific Protocols
WB protocol for FOLR1 antibody 23355-1-APDownload protocol
IHC protocol for FOLR1 antibody 23355-1-APDownload protocol
IF protocol for FOLR1 antibody 23355-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

ACS Nano

Synchronization of Nanoparticle Sensitization and Radiosensitizing Chemotherapy through Cell Cycle Arrest Achieving Ultralow X-ray Dose Delivery to Pancreatic Tumors.

Authors - Divinah Manoharan
Camelus bactrianusWB

Reprod Domest Anim

Screening for reproductive biomarkers in Bactrian camel via iTRAQ analysis of proteomes.

Authors - Qi Wang
humanWB

Nanotechnology

PMID: 28767041

Authors - Yuwei Hu
humanWB

ACS Appl Mater Interfaces

Design and Fabrication of Multifunctional Sericin Nanoparticles for Tumor Targeting and pH-Responsive Subcellular Delivery of Cancer Chemotherapy Drugs.

Authors - Lei Huang
humanWB

J Am Chem Soc

Cancer Selective Target Degradation by Folate-Caged PROTACs.

Authors - Jing Liu
  • KD Validated