Filter: AllWBIHC

WB analysis using 66976-1-Ig (same clone as 66976-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 66976-1-Ig (RBM9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.
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Various lysates were subjected to SDS PAGE followed by western blot with 66976-1-Ig (RBM9 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.
WB analysis of HEK-293 using 66976-1-Ig (same clone as 66976-1-PBS)
WB result of RBM9 antibody (66976-1-Ig; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-RBM9 transfected HEK-293 cells. This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.
WB analysis of HT-29 using 66976-1-Ig (same clone as 66976-1-PBS)
HT-29 cells were subjected to SDS PAGE followed by western blot with 66976-1-Ig (RBM9 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.
WB analysis of NCCIT using 66976-1-Ig (same clone as 66976-1-PBS)
NCCIT cell were subjected to SDS PAGE followed by western blot with 66976-1-Ig (RBM9 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.
WB analysis of fetal human brain using 66976-1-Ig (same clone as 66976-1-PBS)
fetal human brain tissue were subjected to SDS PAGE followed by western blot with 66976-1-Ig (RBM9 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.
WB analysis of human placenta using 66976-1-Ig (same clone as 66976-1-PBS)
human placenta tissue were subjected to SDS PAGE followed by western blot with 66976-1-Ig (RBM9 antibody) at dilution of 1:1600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 66976-1-Ig (same clone as 66976-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 66976-1-Ig (same clone as 66976-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66976-1-Ig (FOX2/RBM9 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.
IHC staining of human pancreas cancer using 66976-1-Ig (same clone as 66976-1-PBS)
Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 66976-1-Ig (FOX2/RBM9 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 66976-1-Ig (same clone as 66976-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:600 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.
IHC staining of human ovary tumor using 66976-1-Ig (same clone as 66976-1-PBS)
Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.
IHC staining of mouse brain using 66976-1-Ig (same clone as 66976-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:600 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 66976-1-Ig (same clone as 66976-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.
IHC staining of human lung cancer using 66976-1-Ig (same clone as 66976-1-PBS)
Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66976-1-Ig (RBM9 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66976-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

FOX2/RBM9 Monoclonal antibody, PBS Only

FOX2/RBM9 Monoclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 66976-1-PBS

Clone No.2D7G5

Review Product

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, IHC, Indirect ELISA

Synonyms

dJ106I20.3, Fox 1 homolog B, Fox 2, FOX2, FOX2/RBM9, fxh, HNRBP2, HRNBP2, RBFOX2, RBM9, RNA binding motif protein 9, RNA binding protein 9, RTA

Formulation: PBS Only

PBS and Azide

PBS Only

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

66976-1-PBS targets FOX2/RBM9 in WB, IHC, Indirect ELISA applications and shows reactivity with Human, Mouse samples.

Tested Reactivity	Human, Mouse
Host / Isotype	Mouse / IgG1
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag28336 Product name: Recombinant human RBM9 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 6-358 aa of BC013115 Sequence: MVTQGNQEPTTTPDAMVQPFTTIPFPPPPQNGIPTEYGVPHTQDYAGQTGEHNLTLYGSTQAHGEQSSNSPSTQNGSLTTEGGAQTDGQQSQTQSSENSESKSTPKRLHVSNIPFRFRDPDLRQMFGGFGFVTFENSADADRAREKLHGTVVEGRKIEVNNATARVMTNKKMVTPYANGWKLSPVVGAVYGPELYAASSFQADVSLGNDAAVPLSGRGGINTYIPLIIPGFPYPTAATTAAAFRGAHLRGRGRTVYGAVRAVPPTAIPAYPGVVYQDGFYGADLYGGYAAYRYAQPATATAATAAAAAVAAYSDGYGRVYTADPYHALAPAASYGVGAVASLYRGGYSRFAPY Predict reactive species
Full Name	RNA binding motif protein 9
Calculated Molecular Weight	390 aa, 41 kDa
Observed Molecular Weight	48 kDa, 50 and 60 kDa
GenBank Accession Number	BC013115
Gene Symbol	RBM9
Gene ID (NCBI)	23543
RRID	AB_2882296
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein G purification
UNIPROT ID	O43251
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

RBM9, also named as RBFOX2 and FOX2, is a RNA-binding protein that regulates alternative splicing events by binding to 5'-UGCAUGU-3' elements. There are several isoforms of RBM9 produced by variable use of multiple alternatively spliced internal exons and alternative splice sites. RBM9 may play an important role in tissue specific alternative splicing regulation.(PMID: 19688295, 21623373, 16537540)