FOXC2 Monoclonal antibody

FOXC2 Monoclonal Antibody for IHC, WB, ELISA

Host / Isotype

Mouse / IgG2b

Reactivity

Human, Rat, Pig and More (1)

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

CloneNo.

3E3E1

Cat no : 68384-1-Ig

Synonyms

FKHL14, Forkhead box protein C2, FOXC2, LD, Mesenchyme fork head protein 1, MFH 1, MFH 1 protein, MFH1, Transcription factor FKH 14

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Validation Data Gallery

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  WB analysis of hTERT-RPE1 using 68384-1-Ig

  hTERT-RPE1 cells were subjected to SDS PAGE followed by western blot with 68384-1-Ig (FOXC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis using 68384-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68384-1-Ig (FOXC2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 68384-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68384-1-Ig (FOXC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 68384-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 68384-1-Ig (FOXC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: hTERT-RPE1 cells, U-87 MG cells, rat kidney tissue, rat heart tissue, rat adipose tissue, pig adipose tissue
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IF: See 1 publications below

Product Information

68384-1-Ig targets FOXC2 in WB, IHC, IF, ELISA applications and shows reactivity with Human, Rat, Pig samples.

• Tested Reactivity: Human, Rat, Pig
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2b
• Class: Monoclonal
• Type: Antibody
• Immunogen: FOXC2 fusion protein Ag19378
• Full Name: forkhead box C2 (MFH-1, mesenchyme forkhead 1)
• Calculated Molecular Weight: 501 aa, 54 kDa
• Observed Molecular Weight: 56 kDa
• GenBank Accession Number: BC113437
• Gene Symbol: FOXC2
• Gene ID (NCBI): 2303
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Forkhead box protein C2 (FOXC2) also known as forkhead-related protein FKHL14 (FKHL14), transcription factor FKH-14, or mesenchyme fork head protein 1 (MFH1) is a protein that in humans is encoded by the FOXC2 gene. FOXC2 is a member of the fork head box (FOX) family of transcription factors. FOX transcription factors are expressed during development and are associated with a number of cellular and developmental differentiation processes. FOXC2 is required during early development of the kidneys, including differentiation of podocytes and maturation of the glomerular basement membrane. It is also involved in the early development of the heart. FOXC2 is also involved in cancer metastases. In particular, expression of FOXC2 is induced when epithelial cells undergo an epithelial-mesenchymal transition (EMT) and become mesenchymal looking cells. (PMID: 8674414 9169153 19935708)

Publications

Species	Application	Title
human	IF	Cell Rep; Stabilizing vimentin phosphorylation inhibits stem-like cell properties and metastasis of hybrid epithelial/mesenchymal carcinomas; Authors - Nick A Kuburich