FOXH1-Specific Polyclonal antibody

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  WB analysis of mouse liver using 20268-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 20268-1-AP (FOXH1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 20268-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 20268-1-AP (FOXH1-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

FOXH1-Specific Polyclonal Antibody for WB, ELISA

Cat No. 20268-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, ELISA

Synonyms

FAST 1, Fast 2, FAST1, FAST2, forkhead box H1, Forkhead box protein H1, FOXH1, hFAST 1

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Tested Applications

• Positive WB detected in: mouse liver tissue, L02 cells

Recommended dilution

• Western Blot (WB): WB : 1:200-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20268-1-AP targets FOXH1-Specific in WB, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: forkhead box H1
• Calculated Molecular Weight: 39 kDa
• Observed Molecular Weight: 40-44 kDa
• GenBank Accession Number: NM_003923
• Gene Symbol: FOXH1
• Gene ID (NCBI): 8928
• RRID: AB_10694676
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: O75593
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FOXH1, also named as FAST1 and FAST2, is a transcriptional activator. FOXH1 recognizes and binds to the DNA sequence 5'-TGT[GT][GT]ATT-3'. It is required for induction of the goosecoid (GSC) promoter by TGF-beta or activin signaling. It forms a transcriptionally active complex containing FOXH1/SMAD2/SMAD4 on a site on the GSC promoter called TARE (TGF-beta/activin response element). The antibody is specific to FOXH1.