Tested Applications
| Positive WB detected in | Caco-2 cells, HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:5000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
28443-1-AP targets FOXJ1 in WB, IF, ELISA applications and shows reactivity with Human samples.
| Tested Reactivity | Human |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag28294 Product name: Recombinant human FOXJ1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 181-421 aa of BC046460 Sequence: KVPREKDEPGKGGFWRIDPQYAERLLSGAFKKRRLPPVHIHPAFARQAAQEPSAVPRAGPLTVNTEAQQLLREFEEATGEAGWGAGEGRLGHKRKQPLPKRVAKVPRPPSTLLPTPEEQGELEPLKGNFDWEAIFDAGTLGGELGALEALELSPPLSPASHVDVDLTIHGRHIDCPATWGPSVEQAADSLDFDETFLATSFLQHPWDESGSGCLPPEPLFEAGDATLASDLQDWASVGAFL Predict reactive species |
| Full Name | forkhead box J1 |
| Calculated Molecular Weight | 45 kDa |
| Observed Molecular Weight | 45-55 kDa |
| GenBank Accession Number | BC046460 |
| Gene Symbol | FOXJ1 |
| Gene ID (NCBI) | 2302 |
| RRID | AB_2918166 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q92949 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FOXJ1 antibody 28443-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FOXJ1 Polyclonal antibody (Cat# 28443-1-AP) has 2 total citations. These appear in Frontiers in Pharmacology and Clinical Respiratory Journal. The cited research focuses on respiratory and cilia biology, specifically examining lung epithelial cilia assembly in severe respiratory infections and Foxj1 down-expression related to impaired cilia architecture in non-cystic fibrosis bronchiectasis. Both studies used the antibody for immunofluorescence (IF) in human samples.
| Species | Application | Title |
|---|---|---|
Front Pharmacol Serum valeric acid stimulates lung epithelial cilia assembly and improves prognosis in patients with severe respiratory infections. | ||
Clin Respir J Down-expression of Foxj1 on airway epithelium with impaired cilia architecture in non-cystic fibrosis bronchiectasis implies disease severity |

