Tested Applications
| Positive WB detected in | mouse ovary tissue, K-562 cells, rat ovary tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19672-1-AP targets FOXL2-Specific in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, pig, chicken |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | forkhead box L2 |
| Calculated Molecular Weight | 39 kDa |
| Observed Molecular Weight | 49 kDa |
| GenBank Accession Number | NM_023067 |
| Gene Symbol | FOXL2 |
| Gene ID (NCBI) | 668 |
| RRID | AB_2878594 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P58012 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FOXL2 is probable a transcriptional regulator. It is required to maintain the female phenotype throughout adulthood, and may provide clues to female infertility. Defects in FOXL2 are a cause of blepharophimosis, ptosis, and epicanthus inversus syndrome (BPES) Defects in FOXL2 are a cause of premature ovarian failure type 3 (POF3). This antibody is specific to the C-term of FOXL2.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FOXL2-Specific antibody 19672-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FOXL2-Specific Polyclonal antibody (Cat# 19672-1-AP) has 17 citations spanning journals including J Adv Res, Cell Death Dis, Clin Cancer Res, FASEB J, Stem Cell Res Ther, and Environ Int. Research fields cover ovarian follicular development, premature ovarian insufficiency, endometriosis, gastric cancer metastasis, sex development disorders, environmental toxicology, and reproductive endocrinology, with applications in WB, IHC, and IF across human, mouse, rat, pig, and chicken species.
| Species | Application | Title |
|---|---|---|
J Adv Res Core fucosylation regulates the ovarian response via FSH receptor during follicular development | ||
J Adv Res Lactational high weight loss impairs follicular development by causing mitochondrial dysfunction of ovarian cells in sows and mitigated by butyrate supplement | ||
Cell Death Dis PARP1-catalyzed PARylation of YY1 mediates endoplasmic reticulum stress in granulosa cells to determine primordial follicle activation | ||
Clin Cancer Res HMGA2-FOXL2 Axis Regulates Metastases and Epithelial-to-mesenchymal Transition of Chemo-resistant Gastric Cancer.
| ||
J Hazard Mater SO2 derivatives impair ovarian function by inhibiting Serpine1/NF-κB pathway-mediated ovarian granulosa cell survival | ||
Environ Int Tracing the epigenetic trajectory of EDC toxicity: Maternal BPAF drives uterine injuries via DNA methylation reprogramming. |
Reviews
What customers say
Both reviewers report positive results. One user (4/5) used the antibody for Western Blot on 293T cells, noting they obtained it through a free promotional offer and were pleased with the outcome. The other (5/5) used it at 1:100 dilution for immunofluorescence on mouse ovary tissue, describing it as an excellent granulosa cell marker that clearly distinguished granulosa cells (FOXL2-positive) from germ cells (TRA98-positive) in post-natal day 2 samples. Overall, users find this antibody reliable and effective for its intended applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Y C (Verified Customer) (01-23-2020) | I use the free trial size antibody code from the 12 days of free X'mas gifts to order this.Free product and one figure!! Yay!
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Kathryn (Verified Customer) (11-04-2019) | Image shows post-natal day 2 mouse ovary with germ cells marked by TRA98 (Abcam) in green and granulosa cells marked by FOXL2 (Proteintech) in red. This is an excellent granulosa cell marker.
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