FOXO4 Polyclonal antibody

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  WB analysis using 21535-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21535-1-AP (FOXO4 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21535-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21535-1-AP (FOXO4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 21535-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21535-1-AP (FOXO4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 21535-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 21535-1-AP (FOXO4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• Featured Product
• KD/KO Validated

FOXO4 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 21535-1-AP

Publications(23)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (2)

Applications

WB, IHC, IF, CoIP, ELISA

Synonyms

AFX, AFX1, forkhead box O4, Forkhead box protein O4, FOXO4, MLLT7

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Tested Applications

• Positive WB detected in: PC-12 cells, mouse heart tissue, mouse kidney tissue, rat kidney tissue
• Positive IHC detected in: human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 20 publications below
• IHC: See 6 publications below
• IF: See 4 publications below
• CoIP: See 1 publications below

Product Information

21535-1-AP targets FOXO4 in WB, IHC, IF, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat, canine, bovine
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag16169

  Product name: Recombinant human FOXO4 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 198-505 aa of BC106761

  Sequence: MDSSSKLLRGRSKAPKKKPSVLPAPPEGATPTSPVGHFAKWSGSPCSRNREEADMWTTFRPRSSSNASSVSTRLSPLRPESEVLAEEIPASVSSYAGGVPPTLNEGLELLDGLNLTSSHSLLSRSGLSGFSLQHPGVTGPLHTYSSSLFSPAEGPLSAGEGCFSSSQALEALLTSDTPPPPADVLMTQVDPILSQAPTLLLLGGLPSSSKLATGVGLCPKPLEAPGPSSLVPTLSMIAPPPVMASAPIPKALGTPVLTPPTEAASQDRMPQDLDLDMYMENLECDMDNIISDLMDEGEGLDFNFEPDP

• Full Name: forkhead box O4
• Calculated Molecular Weight: 505 aa, 54 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC106761
• Gene Symbol: FOXO4
• Gene ID (NCBI): 4303
• RRID: AB_10732960
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P98177
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FOXO4, also named as AFX, AFX1 and MLLT7, is a transcription factor which involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1.FOXO down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is involved in negative regulation of the cell cycle. A chromosomal aberration involving FOXO4 is found in acute leukemias. The antibody can recognize isoform 1 and isoform Zeta of FOXO4.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
canine	WB	Circ Res; Suppression of Activated FOXO Transcription Factors in the Heart Prolongs Survival in a Mouse Model of Laminopathies.; Authors - Gaelle Auguste
human	WB	J Clin Invest; Hepatitis B virus X protein represses miRNA-148a to enhance tumorigenesis.; Authors - Xu Xiaojie X ; KD Validated
mouse,rat	WB,IHC	Nat Commun; Damaged brain accelerates bone healing by releasing small extracellular vesicles that target osteoprogenitors.; Authors - Wei Xia
rat	WB	Redox Biol; Notoginsenoside R1-loaded mesoporous silica nanoparticles targeting the site of injury through inflammatory cells improves heart repair after myocardial infarction.; Authors - Han Li
human	IF	Mater Today Bio; Ultra-small polydopamine nanomedicine-enabled antioxidation against senescence; Authors - Jiamei Han
rat	WB	J Agric Food Chem; Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.; Authors - Shu Yang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

zhiping (Verified Customer) (06-04-2021)

It works pretty well for IHC.

• Applications: Immunohistochemistry
• Cell Tissue Type: human skin