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  • KD/KO Validated

FOXO4 Polyclonal antibody

FOXO4 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG


human, mouse, rat and More (2)





Cat no : 21535-1-AP


AFX, AFX1, forkhead box O4, Forkhead box protein O4, FOXO4, MLLT7

Tested Applications

Positive WB detected inPC-12 cells, mouse heart tissue, mouse kidney tissue, rat kidney tissue
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

The immunogen of 21535-1-AP is FOXO4 Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, canine, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FOXO4 fusion protein Ag16169
Full Name forkhead box O4
Calculated Molecular Weight 505 aa, 54 kDa
Observed Molecular Weight 65 kDa
GenBank Accession NumberBC106761
Gene Symbol FOXO4
Gene ID (NCBI) 4303
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FOXO4, also named as AFX, AFX1 and MLLT7, is a transcription factor which involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1.FOXO down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is involved in negative regulation of the cell cycle. A chromosomal aberration involving FOXO4 is found in acute leukemias. The antibody can recognize isoform 1 and isoform Zeta of FOXO4.


Product Specific Protocols
WB protocol for FOXO4 antibody 21535-1-APDownload protocol
IHC protocol for FOXO4 antibody 21535-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols



Circ Res

Suppression of Activated FOXO Transcription Factors in the Heart Prolongs Survival in a Mouse Model of Laminopathies.

Authors - Gaelle Auguste

J Clin Invest

Hepatitis B virus X protein represses miRNA-148a to enhance tumorigenesis.

Authors - Xu Xiaojie X
  • KD Validated

Nat Commun

Damaged brain accelerates bone healing by releasing small extracellular vesicles that target osteoprogenitors.

Authors - Wei Xia

Redox Biol

Notoginsenoside R1-loaded mesoporous silica nanoparticles targeting the site of injury through inflammatory cells improves heart repair after myocardial infarction.

Authors - Han Li

Mater Today Bio

Ultra-small polydopamine nanomedicine-enabled antioxidation against senescence

Authors - Jiamei Han

J Agric Food Chem

Mitotically stable modification of DNA methylation in IGF2/H19 imprinting control region is associated with activated hepatic IGF2 expression in offspring rats from betaine-supplemented dams.

Authors - Shu Yang


The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


zhiping (Verified Customer) (06-04-2021)

It works pretty well for IHC.

  • Applications: Immunohistochemistry
  • Cell Tissue Type: human skin