Tested Applications
| Positive WB detected in | C2C12 cells, mouse brain tissue, mouse lung tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | mouse brain tissue, human gliomas tissue, human brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
19122-1-AP targets FOXO6 in WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | forkhead box protein O6 |
| Calculated Molecular Weight | 51 kDa |
| Observed Molecular Weight | 70-80 kDa |
| GenBank Accession Number | AI361654 |
| Gene Symbol | FOXO6 |
| Gene ID (NCBI) | 100132074 |
| RRID | AB_10597247 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | A8MYZ6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FOXO6 is a member of the murine forkhead family of transcription factors. These forkhead transcription factors may play a role in maintenance. FOXO6 mRNA is predominantly expressed in the developing brain, indicating that FOXO6 may play an important role in the nervous system. FOXO6 has an isoform of 70 kDa when modified after translation (PMID:23639108).
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FOXO6 antibody 19122-1-AP | Download protocol |
| IHC protocol for FOXO6 antibody 19122-1-AP | Download protocol |
| IP protocol for FOXO6 antibody 19122-1-AP | Download protocol |
| WB protocol for FOXO6 antibody 19122-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
FOXO6 Polyclonal Antibody (Cat# 19122-1-AP) has 21 citations across 20 journals, including Theranostics, Acta Pharm Sin B, Cell Death Dis, Oncotarget, FASEB J, and Elife. Research fields span oncology (gastric, breast, liver, lung, ovarian cancers), cardiovascular disease, neuroinflammation, ferroptosis, autophagy, metabolic regulation, and gene regulation. Cited applications include WB, IHC, IF, CoIP, and ChIP across human, mouse, rat, and pig species.
| Species | Application | Title |
|---|---|---|
Theranostics An anti-FAP-scFv-functionalized exosome-carrying hydrogel delivers SKI mRNA to fibrotic nucleus pulposus cells to alleviate intervertebral disc degeneration by regulating FOXO3 | ||
Acta Pharm Sin B USP25 ameliorates vascular remodeling by deubiquitinating FOXO3 and promoting autophagic degradation of FOXO3 | ||
MedComm (2020) Forkhead box O6 (FoxO6) promotes cardiac pathological remodeling and dysfunction by activating Kif15-TGF-β1 under aggravated afterload | ||
Cell Death Dis SGK2 promotes prostate cancer metastasis by inhibiting ferroptosis via upregulating GPX4 | ||
Cell Death Discov PLOD3 contributes to HER-2 therapy resistance in gastric cancer through FoxO3/Survivin pathway. | ||
Arterioscler Thromb Vasc Biol Inflammatory Cell-Derived MYDGF Attenuates Endothelial LDL Transcytosis to Protect Against Atherogenesis |
Reviews
What customers say
The single review for this antibody (used in Western Blot at 1:2000 on 293T overexpression samples) reports that it detects mouse Foxo1 easily but shows cross-reactivity with Foxo1, and the reviewer rated it 1 star. Overall, the feedback highlights strong detection of the target while noting off-target binding that may limit specificity in some applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Young-Wook (Verified Customer) (10-31-2017) | This antibody detect easily mouse Foxo1 than mouse Foxo6. There is cross reactivity with Foxo1.
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