FOXO6 Recombinant antibody

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  WB analysis using 83926-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83926-1-RR (FOXO6 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IHC staining of rat brain using 83926-1-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 83926-1-RR (FOXO6 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of rat brain using 83926-1-RR

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using FOXO6 antibody (83926-1-RR, Clone: 240823D10 ) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 83926-1-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using FOXO6 antibody (83926-1-RR, Clone: 240823D10 ) at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

FOXO6 {ptg:Brand} Recombinant Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Cat No. 83926-1-RR

Clone No.240823D10

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Synonyms

forkhead box protein O6, 240823D10

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Tested Applications

• Positive WB detected in: HeLa cells, HepG2 cells
• Positive IHC detected in: rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF-P detected in: rat brain tissue
• Positive IF/ICC detected in: A549 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochemistry (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

83926-1-RR targets FOXO6 in WB, IHC, IF/ICC, IF-P, ELISA applications and shows reactivity with human, rat samples.

• Tested Reactivity: human, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: Peptide
• Full Name: forkhead box protein O6
• Calculated Molecular Weight: 51 kDa
• Observed Molecular Weight: 75 kDa
• GenBank Accession Number: AI361654
• Gene Symbol: FOXO6
• Gene ID (NCBI): 100132074
• RRID: AB_3671506
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol , pH 7.3
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FOXO6 is a member of the murine forkhead family of transcription factors. These forkhead transcription factors may play a role in maintenance. FOXO6 mRNA is predominantly expressed in the developing brain, indicating that FOXO6 may play an important role in the nervous system.