Tested Applications
| Positive WB detected in | HCT 116 cells, Neuro-2a cells, mouse brain tissue, mouse lung tissue, rat brain tissue |
| Positive IP detected in | mouse spleen tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 2 publications below |
| IHC | See 4 publications below |
| IF | See 2 publications below |
| CoIP | See 1 publications below |
Product Information
20529-1-AP targets FOXP2 in WB, IHC, IF, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14391 Product name: Recombinant human FOXP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-56 aa of BC018016 Sequence: MMQESATETISNSSMNQNGMSTLSSQLDAGSRDGRSSGDTSSEVSTVELLHLQQQQ Predict reactive species |
| Full Name | forkhead box P2 |
| Calculated Molecular Weight | 80 kDa |
| Observed Molecular Weight | 70-80 kDa |
| GenBank Accession Number | BC018016 |
| Gene Symbol | FOXP2 |
| Gene ID (NCBI) | 93986 |
| RRID | AB_10695756 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15409 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FOXP2, Forkhead box protein P2, is associated with speech and language development (PMID: 24765219). FOXP2 is expressed in the brain, lungs, heart and gut. In the brain, it is widely expressed in sensory, limbic and motor structures(PMID: 10880297, PMID:15685218). Mutations in FOXP2 caused the severe speech and language disorder developmental verbal dyspraxia (PMID: 15877281). FOXP2 was detected 2 isoforms of 70 kDa and 80-82 kDa (PMID: 15056696, PMID: 29360038, PMID: 17999357).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for FOXP2 antibody 20529-1-AP | Download protocol |
| WB protocol for FOXP2 antibody 20529-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Elife FoxP2 isoforms delineate spatiotemporal transcriptional networks for vocal learning in the zebra finch. | ||
Mol Ther Nucleic Acids circ-SHKBP1 Regulates the Angiogenesis of U87 Glioma-Exposed Endothelial Cells through miR-544a/FOXP1 and miR-379/FOXP2 Pathways. | ||
J Cancer Low expression of FOXP2 predicts poor survival and targets caspase-1 to inhibit cell pyroptosis in colorectal cancer.
| ||
BMC Biol GnRH-driven FTO-mediated RNA m6A modification promotes gonadotropin synthesis and secretion | ||
Commun Biol FOXP2 overexpression upregulates LAMA4 expression and thereby alleviates preeclampsia by regulating trophoblast behavior | ||
Cell Rep The endocytic adaptor AP-2 maintains Purkinje cell function by balancing cerebellar parallel and climbing fiber synapses |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Reyes (Verified Customer) (10-18-2024) | FOXP2 (in green) worked marking my neurons (in red) in FFPE human brain tissue, although other tests to check the specificity of the layer need to be performed.
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