Tested Applications
| Positive WB detected in | HCT 116 cells, Neuro-2a cells, mouse brain tissue, mouse lung tissue, rat brain tissue |
| Positive IP detected in | mouse spleen tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20529-1-AP targets FOXP2 in WB, IHC, IF, IP, CoIP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14391 Product name: Recombinant human FOXP2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-56 aa of BC018016 Sequence: MMQESATETISNSSMNQNGMSTLSSQLDAGSRDGRSSGDTSSEVSTVELLHLQQQQ Predict reactive species |
| Full Name | forkhead box P2 |
| Calculated Molecular Weight | 80 kDa |
| Observed Molecular Weight | 70-80 kDa |
| GenBank Accession Number | BC018016 |
| Gene Symbol | FOXP2 |
| Gene ID (NCBI) | 93986 |
| RRID | AB_10695756 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O15409 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FOXP2, Forkhead box protein P2, is associated with speech and language development (PMID: 24765219). FOXP2 is expressed in the brain, lungs, heart and gut. In the brain, it is widely expressed in sensory, limbic and motor structures(PMID: 10880297, PMID:15685218). Mutations in FOXP2 caused the severe speech and language disorder developmental verbal dyspraxia (PMID: 15877281). FOXP2 was detected 2 isoforms of 70 kDa and 80-82 kDa (PMID: 15056696, PMID: 29360038, PMID: 17999357).
Protocols
| Product Specific Protocols | |
|---|---|
| IP protocol for FOXP2 antibody 20529-1-AP | Download protocol |
| WB protocol for FOXP2 antibody 20529-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
FOXP2 Polyclonal antibody (Cat# 20529-1-AP) has 10 citations across journals including Nature Chemical Biology, Cell Reports, Molecular Therapy Nucleic Acids, Communications Biology, Biology Direct, BMC Biology, iScience, Applied Biochemistry and Biotechnology, Journal of Cancer, and eLife. Research fields span neurobiology (speech development, vocal learning, Purkinje cell function), oncology (glioma, hepatocellular carcinoma, colorectal cancer), reproductive biology (preeclampsia, gonadotropin secretion), RNA m6A modification, oxidative stress, and angiogenesis.
| Species | Application | Title |
|---|---|---|
Nat Chem Biol Designing chemigenetic DNA nanotrap for norepinephrine dynamic imaging in organelles. | ||
Cell Rep The endocytic adaptor AP-2 maintains Purkinje cell function by balancing cerebellar parallel and climbing fiber synapses | ||
Mol Ther Nucleic Acids circ-SHKBP1 Regulates the Angiogenesis of U87 Glioma-Exposed Endothelial Cells through miR-544a/FOXP1 and miR-379/FOXP2 Pathways. | ||
Commun Biol FOXP2 overexpression upregulates LAMA4 expression and thereby alleviates preeclampsia by regulating trophoblast behavior | ||
Biol Direct Unraveling the oxidative stress landscape in diabetic foot ulcers: insights from bulk RNA and single-cell RNA sequencing data | ||
BMC Biol GnRH-driven FTO-mediated RNA m6A modification promotes gonadotropin synthesis and secretion |
Reviews
What customers say
The single review reports using this antibody for immunofluorescence on FFPE human brain cortex at a 1:100 dilution. The user observed clear FOXP2 signal (green) marking neurons (red), though they noted additional specificity tests were still needed to confirm layer-specific labeling. The product received a 4-star rating.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Reyes (Verified Customer) (10-18-2024) | FOXP2 (in green) worked marking my neurons (in red) in FFPE human brain tissue, although other tests to check the specificity of the layer need to be performed.
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