Tested Applications
| Positive WB detected in | A549 cells, HeLa cells, Jurkat cells |
| Positive IP detected in | mouse lung tissue |
| Positive IHC detected in | human thyroid cancer tissue, human ovary tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells |
| Positive FC (Intra) detected in | Jurkat cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:400-1:1600 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16772-1-AP targets FOXP4 in WB, IHC, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10047 Product name: Recombinant human FOXP4 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 327-678 aa of BC052803 Sequence: NTEHALDDRSTAQCRVQMQVVQQLEIQLAKESERLQAMMAHLHMRPSEPKPFSQPLNPVPGSSSFSKVTVSAADSFPDGLVHPPTSAAAPVTPLRPPGLGSASLHGGGPARRRSSDKFCSPISSELAQNHEFYKNADVRPPFTYASLIRQAILETPDRQLTLNEIYNWFTRMFAYFRRNTATWKNAVRHNLSLHKCFVRVENVKGAVWTVDEREYQKRRPPKMTGSPTLVKNMISGLSYGALNASYQAALAESSFPLLNSPGMLNPGSASSLLPLSHDDVGAPVEPLPSNGSSSPPRLSPPQYSHQVQVKEEPAEAEEDRQPGPPLGAPNPSASGPPEDRDLEEELPGEELS Predict reactive species |
| Full Name | forkhead box P4 |
| Calculated Molecular Weight | 678 aa, 73 kDa |
| Observed Molecular Weight | 73 kDa |
| GenBank Accession Number | BC052803 |
| Gene Symbol | FOXP4 |
| Gene ID (NCBI) | 116113 |
| RRID | AB_2262825 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IVH2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for FOXP4 antibody 16772-1-AP | Download protocol |
| IF protocol for FOXP4 antibody 16772-1-AP | Download protocol |
| IHC protocol for FOXP4 antibody 16772-1-AP | Download protocol |
| IP protocol for FOXP4 antibody 16772-1-AP | Download protocol |
| WB protocol for FOXP4 antibody 16772-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FOXP4 Polyclonal antibody (Cat# 16772-1-AP) has 7 citations published in Cell Death & Disease, Communications Biology, Molecular Medicine Reports, Cancer Science, Journal of Lipid Research, Cancer Medicine, and Heliyon. Research fields span colorectal cancer metastasis, endometrial carcinoma, airway fibrosis and EMT, cervical intraepithelial neoplasia, adipocyte thermogenic regulation, and thyroid cancer progression.
| Species | Application | Title |
|---|---|---|
Cell Death Dis FOXP4 promotes metastatic progression in colorectal cancer through transcriptional activation of BAG3. | ||
Mol Med Rep Downregulation of microRNA‑423‑5p suppresses TGF‑β1‑induced EMT by targeting FOXP4 in airway fibrosis. | ||
J Lipid Res The Forkhead Box Transcription Factor FoxP4 Regulates Thermogenic Programs in Adipocytes.
| ||
Cancer Sci FOXP4 inhibits squamous differentiation of atypical cells in cervical intraepithelial neoplasia via an ELF3-dependent pathway | ||
Cancer Med Androgen promotes squamous differentiation of atypical cells in cervical intraepithelial neoplasia via an ELF3-dependent pathway |
