FOXQ1 Polyclonal antibody

• Featured Product
• KD/KO Validated

FOXQ1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 23718-1-AP

Synonyms

HNF 3/forkhead like protein 1, HFH-1, HFH1, HFH 1, forkhead box Q1

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Validation Data Gallery

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  WB analysis of mouse kidney using 23718-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23718-1-AP (FOXQ1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of rat kidney using 23718-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 23718-1-AP (FOXQ1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human stomach cancer using 23718-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 23718-1-AP (FOXQ1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse kidney tissue, rat kidney tissue
• Positive IHC detected in: human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

23718-1-AP targets FOXQ1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FOXQ1 fusion protein Ag20545
• Full Name: forkhead box Q1
• Calculated Molecular Weight: 403 aa, 41 kDa
• Observed Molecular Weight: 42 kDa
• GenBank Accession Number: BC053850
• Gene Symbol: FOXQ1
• Gene ID (NCBI): 94234
• RRID: AB_2879310
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen Affinity purified
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Forkhead box Q1 (FOXQ1, also known as HFH1), a member of the FOX gene family, contains the core DNA binding domain, whereas the flanking wings of FOXQ1 contribute to its sequence specificity. FOXQ1 is known to repress the promoter activity of smooth muscle-specific genes, such as telokin and SM22α. FOXQ1 expression is regulated by Hoxa1 in embryonic stem cells. [PMID:15722554, 8139574] As a transcription factor, FOXQ1 represses the promoter activity of smooth muscle-specific genes, such as telokin and SM22α, in A10 vascular muscle cells [PMID:10896677].

Publications

Species	Application	Title
mouse,human	WB,IHC,IF,IP	Cell Death Dis; FOXQ1 promotes pancreatic cancer cell proliferation, tumor stemness, invasion and metastasis through regulation of LDHA-mediated aerobic glycolysis; Authors - Changhao Wu ; KD Validated
human	WB	Cell Death Dis; Phosphorylated FOXQ1, a novel substrate of JNK1, inhibits sorafenib-induced ferroptosis by activating ETHE1 in hepatocellular carcinoma; Authors - Yiwei Liu
mouse	IF	Am J Respir Cell Mol Biol; Foxq1 Promotes Alveolar Epithelial Cell Death through Tle1-mediated Inhibition of the NFκB Signaling Pathway; Authors - Xi Zhu
human	WB	Breast Cancer Res; Long noncoding RNA PVT1 promotes breast cancer proliferation and metastasis by binding miR-128-3p and UPF1.; Authors - Shuiyi Liu
human	WB	Front Oncol; The Novel lncRNA RP9P Promotes Colorectal Cancer Progression by Modulating miR-133a-3p/FOXQ1 Axis.; Authors - Zhichao Jin ; KD Validated
human	WB	Mol Carcinog; Monocarboxylate transporter 1 is a novel target for breast cancer stem like-cell inhibition by diallyl trisulfide.; Authors - Su-Hyeong Kim