FPGS Polyclonal antibody

• 

  WB analysis using 27616-1-AP

  HeLa mitochondrial extracts were subjected to SDS PAGE followed by western blot with 27616-1-AP (FPGS antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 27616-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27616-1-AP (FPGS antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

FPGS Polyclonal Antibody for WB, ELISA

Cat No. 27616-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

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Tested Applications

• Positive WB detected in: HeLa mitochondrial extract

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

27616-1-AP targets FPGS in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FPGS fusion protein Ag24775
• Full Name: folylpolyglutamate synthase
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC064393
• Gene Symbol: FPGS
• Gene ID (NCBI): 2356
• RRID: AB_3669615
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q05932
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FPGS is located in the cytosol and mitochondria of mammalian tissues, with current evidence supporting a single gene encoding for both the cytosolic and mitochondrial isoenzymes. FPGS catalyzes a rate-limiting polyglutamylation step in the folic acid metabolic pathway and increases the intracellular retention of folates (PMID: 8907263, PMID: 31611592). FPGS has 4 isoforms with the molecular mass of 59-65 kDa.