Tested Applications
| Positive WB detected in | mouse brain tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
29654-1-AP targets FRAS1 in WB, ELISA applications and shows reactivity with Human, mouse samples.
| Tested Reactivity | Human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag31229 Product name: Recombinant human FRAS1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 242-388 aa of NM_025074 Sequence: CICDRGEVRCHKQACLPLRCGKGQSRARRHGQCCEECVSPAGSCSYDGVVRYQDEMWKGSACEFCMCDHGQVTCQTGECAKVECARDEELIHLDGKCCPECISRNGYCVYEETGEFMSSNASEVKRIPEGEKWEDGPCKVCECRGAQ Predict reactive species |
| Full Name | Fraser syndrome 1 |
| Calculated Molecular Weight | 443 kDa |
| Observed Molecular Weight | 450-500 kDa |
| GenBank Accession Number | NM_025074 |
| Gene Symbol | FRAS1 |
| Gene ID (NCBI) | 80144 |
| RRID | AB_3086149 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q86XX4 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FRAS1 (extracellular matrix organizing protein FRAS1) is required for the regulation of epidermal-basement membrane adhesion responsible for proper organogenesis during embryonic development, and it is also involved in brain organization and function.
Protocols
| Product Specific Protocols | |
|---|---|
| WB protocol for FRAS1 antibody 29654-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Evangelos (Verified Customer) (06-17-2024) | Excellent antibody for detecting the very large FRAS1 protein in western blot! We used it at a dilution of 1:5000, to detect FRAS1 in prostate cancer cell lysates and woked out great. We confirmed specificity by comparing parental vs. FRAS1 knockout and knock down cells. Highly recomend it
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