FRK Polyclonal antibody

FRK Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 16197-1-AP

Synonyms

FRK, fyn related kinase, GTK, Protein tyrosine kinase 5, PTK5, RAK, Tyrosine protein kinase FRK



Tested Applications

Positive WB detected inHEK-293 cells, MCF-7 cells, mouse kidney tissue, K-562 cells, NIH/3T3 cells
Positive IHC detected inhuman breast cancer tissue, human bladder tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHepG2 cells
Positive FC detected inHepG2 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 16197-1-AP is FRK Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FRK fusion protein Ag10372
Full Name fyn-related kinase
Calculated molecular weight 505 aa, 56 kDa
Observed molecular weight 54-57 kDa
GenBank accession numberBC012916
Gene symbol FRK
Gene ID (NCBI) 2444
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

The fyn-related kinase (FRK) belongs to the tyrosine kinase family of protein kinases and is also named as PTK5(protein-tyrosine kinase 5), RAK(Nuclear tyrosine protein kinase). The Frk is a tyrosine kinase expressed in various tissues, such as the gastrointestinal tract and pancreatic islet cells. It affects pancreatic beta cell number during embryogenesis and promotes beta cell cytotoxic signals in response to streptozotocin(PMID:20157399). It may function as a tumor suppressor(PMID:19345329).

Protocols

Product Specific Protocols
WB protocol for FRK antibody 16197-1-APDownload protocol
IHC protocol for FRK antibody 16197-1-APDownload protocol
IF protocol for FRK antibody 16197-1-APDownload protocol
FC protocol for FRK antibody 16197-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols