FRMD7 Polyclonal antibody

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• FRMD7 Polyclonal antibody (25033-1-AP)

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  WB analysis using 25033-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25033-1-AP (FRMD7 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

FRMD7 Polyclonal Antibody for WB, ELISA

Cat No. 25033-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

FERM domain containing 7, NYS, NYS1

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Tested Applications

• Positive WB detected in: SH-SY5Y cells, SK-N-SH cells, Neuro-2a cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

25033-1-AP targets FRMD7 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag18161

  Product name: Recombinant human FRMD7 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 385-714 aa of BC114371

  Sequence: LEVTFATELEHSKPEADPTLLHQSQSSSSFPFIYMDPVFNTEPNPNPDPRDIFSERSSLSSFQTSCKFSGNHMSIYSGLTSKVRPAKQLTYTDVPYIPCTGQQVGIMPPQVFFYVDKPPQVPRWSPIRAEERTSPHSYVEPTAMKPAERSPRNIRMKSFQQDLQVLQEAIARTSGRSNINVGLEEEDPNLEDAFVCNIQEQTPKRSQSQSDMKTIRFPFGSEFRPLGPCPALSHKADLFTDMFAEQELPAVLMDQSTAERYVASESSDSESEILKPDYYALYGKEIRSPMARIRLSSGSLQLDEEDEDAYFNTPTAEDRTSLKPCNYFLA

• Full Name: FERM domain containing 7
• Calculated Molecular Weight: 714 aa, 82 kDa
• Observed Molecular Weight: 80-90 kDa
• GenBank Accession Number: BC114371
• Gene Symbol: FRMD7
• Gene ID (NCBI): 90167
• RRID: AB_3742133
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6ZUT3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

The 714-amino acid FRMD7 protein contains an N-terminal FERM domain and FERM-adjacent (FA) domain. FRMD7 shares close homology with FARP1 (FERM, RhoGEF, and pleckstrin domain protein 1) as well as FARP2. FRMD7 also promotes neurite elongation at the actin-rich growth cone ends through the modulation of the actin cytoskeleton.