FSHR Polyclonal antibody

FSHR Polyclonal Antibody for FC, IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse and More (5)

Applications

WB, IHC, IF, FC,ELISA

Conjugate

Unconjugated

Cat no : 22665-1-AP

Synonyms

Follitropin receptor, FSH R, FSHR, FSHRO, LGR1, ODG1



Tested Applications

Positive WB detected inDU 145 cells, HeLa cells, PC-3 cells
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells
Positive FC detected inHeLa cells
Planning an IF experiment? We recommend our CoraLite®594 conjugated versions of this antibody.

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 22665-1-AP is FSHR Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity bovine, chicken, geese, human, mouse, rat, Sheep
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FSHR fusion protein Ag18158
Full Name follicle stimulating hormone receptor
Calculated molecular weight 695 aa, 78 kDa
Observed molecular weight 71-78 kDa
GenBank accession numberBC118548
Gene symbol FSHR
Gene ID (NCBI) 2492
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for FSHR antibody 22665-1-APDownload protocol
IHC protocol for FSHR antibody 22665-1-APDownload protocol
IF protocol for FSHR antibody 22665-1-APDownload protocol
FC protocol for FSHR antibody 22665-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIF

Hum Reprod

Estrogen receptor β upregulates CCL2 via NF-κB signaling in endometriotic stromal cells and recruits macrophages to promote the pathogenesis of endometriosis.

Authors - Yanling Gou
bovineIF

Environ Toxicol Pharmacol

Melatonin alleviates β-zearalenol and HT-2 toxin-induced apoptosis and oxidative stress in bovine ovarian granulosa cells.

Authors - Fangxiao Yang
humanIF,FC

Mol Cell Endocrinol

Novel FSHR mutations in Han Chinese women with sporadic premature ovarian insufficiency.

Authors - Hongli Liu
WB

J Assist Reprod Genet

Molecular mechanism of FSHR expression induced by BMP15 in human granulosa cells.

Authors - Ken Shimizu
mouseIF

Ecotoxicol Environ Saf

Chlorogenic acid rescues zearalenone induced injury to mouse ovarian granulosa cells.

Authors - YanYan Yi
geeseIF

Anim Biotechnol

MiR-34c-5p promotes granulosa cells apoptosis by targeting Bcl2 in broody goose (Anser cygnoides).

Authors - Li'e Hou