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FSHR Polyclonal antibody

FSHR Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB,IHC,IF,FC,ELISA

Conjugate

Unconjugated

Cat no : 22665-1-AP

Synonyms

Follitropin receptor, FSH R, FSHR, FSHRO, LGR1, ODG1



Tested Applications

Positive WB detected inmouse ovary tissue, rat ovary tissue, DU 145 cells, PC-3 cells, HeLa cells
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22665-1-AP targets FSHR in WB,IHC,IF,FC,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat, chicken, Sheep, geese, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FSHR fusion protein Ag18158
Full Name follicle stimulating hormone receptor
Calculated Molecular Weight 695 aa, 78 kDa
Observed Molecular Weight 71-78 kDa
GenBank Accession NumberBC118548
Gene Symbol FSHR
Gene ID (NCBI) 2492
RRIDAB_2631204
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FSHR antibody 22665-1-APDownload protocol
IHC protocol for FSHR antibody 22665-1-APDownload protocol
IF protocol for FSHR antibody 22665-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Res

Blocking FSH inhibits hepatic cholesterol biosynthesis and reduces serum cholesterol.

Authors - Yanjing Guo
human,mouseWB

Autophagy

Autophagy regulates differentiation of ovarian granulosa cells through degradation of WT1

Authors - Tong Shao
mouseIHC

Redox Biol

Novel ovarian endometriosis model causes infertility via iron-mediated oxidative stress in mice.

Authors - Shotaro Hayashi
humanIF

ACS Appl Mater Interfaces

A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells

Authors - Xinyang Zhao
human,mouseWB,IHC

Free Radic Biol Med

Iron overload modulates follicular microenvironment via ROS/HIF-1α/FSHR signaling

Authors - Yaoqiu Wu
mouseIF

Ecotoxicol Environ Saf

Chlorogenic acid rescues zearalenone induced injury to mouse ovarian granulosa cells.

Authors - YanYan Yi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Nolan (Verified Customer) (04-11-2022)

Antibody works better if you stain at room temperature for 2 hours instead of 4C overnight.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse FSHR