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FSHR Polyclonal antibody

FSHR Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (5)

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Cat no : 22665-1-AP

Synonyms

Follitropin receptor, FSH R, FSHR, FSHRO, LGR1, ODG1



Tested Applications

Positive WB detected inmouse ovary tissue, rat ovary tissue, DU 145 cells, PC-3 cells, HeLa cells
Positive IHC detected inmouse ovary tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHeLa cells, HepG2 cells
Positive FC detected inHeLa cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:50-1:500
Flow Cytometry (FC)FC : 0.20 ug per 10^6 cells in a 100 µl suspension
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

22665-1-AP targets FSHR in WB, IHC, IF, FC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman, mouse, rat, goat, chicken, Sheep, geese, bovine
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FSHR fusion protein Ag18158
Full Name follicle stimulating hormone receptor
Calculated Molecular Weight 695 aa, 78 kDa
Observed Molecular Weight 71-78 kDa
GenBank Accession NumberBC118548
Gene Symbol FSHR
Gene ID (NCBI) 2492
RRIDAB_2631204
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for FSHR antibody 22665-1-APDownload protocol
IHC protocol for FSHR antibody 22665-1-APDownload protocol
IF protocol for FSHR antibody 22665-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseIF

Cell Res

Blocking FSH inhibits hepatic cholesterol biosynthesis and reduces serum cholesterol.

Authors - Yanjing Guo
human,mouseWB

Autophagy

Autophagy regulates differentiation of ovarian granulosa cells through degradation of WT1

Authors - Tong Shao
mouseIHC

Redox Biol

Novel ovarian endometriosis model causes infertility via iron-mediated oxidative stress in mice.

Authors - Shotaro Hayashi
humanIF

ACS Appl Mater Interfaces

A Novel Three-Dimensional Follicle Culture System Decreases Oxidative Stress and Promotes the Prolonged Culture of Human Granulosa Cells

Authors - Xinyang Zhao
human,mouseWB,IHC

Free Radic Biol Med

Iron overload modulates follicular microenvironment via ROS/HIF-1α/FSHR signaling

Authors - Yaoqiu Wu
mouseIF

Ecotoxicol Environ Saf

Chlorogenic acid rescues zearalenone induced injury to mouse ovarian granulosa cells.

Authors - YanYan Yi

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Nolan (Verified Customer) (04-11-2022)

Antibody works better if you stain at room temperature for 2 hours instead of 4C overnight.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:500
  • Cell Tissue Type: Mouse FSHR