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WB analysis using 28218-1-AP
Human placenta tissues were subjected to SDS PAGE followed by western blot with 28218-1-AP (FSTL3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.
WB analysis of HeLa using 28218-1-AP
WB result of FSTL3 antibody (28218-1-AP; 1:400; incubated at room temperature for 1.5 hours) with sh-Control and sh-FSTL3 transfected HeLa cells.
IHC staining of human urothelial carcinoma using 28218-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 28218-1-AP (FSTL3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of human urothelial carcinoma using 28218-1-AP
Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 28218-1-AP (FSTL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 28218-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 28218-1-AP (FSTL3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat kidney using 28218-1-AP
Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 28218-1-AP (FSTL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat testis using 28218-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28218-1-AP (FSTL3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).
IHC staining of rat testis using 28218-1-AP
Immunohistochemical analysis of paraffin-embedded rat testis tissue slide using 28218-1-AP (FSTL3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Featured Product
KD/KO Validated

FSTL3/FLRG Polyclonal antibody

Name: FSTL3/FLRG Polyclonal antibody
Brand: Proteintech
SKU: 28218-1-AP

FSTL3/FLRG Polyclonal Antibody for WB, IHC, ELISA

Cat No. 28218-1-AP

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, rat

Applications

WB, IHC, ELISA

Synonyms

FLRG, FSTL3, Follistatin-related protein 3, Follistatin-related gene protein, Follistatin-like protein 3

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

Conjugate: Unconjugated

Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in	human placenta tissue, HeLa cells
Positive IHC detected in	human urothelial carcinoma tissue, rat kidney tissue, rat testis tissue **Note: suggested antigen retrieval with TE buffer pH 9.0; () Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0***

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:500-1:1000
Immunohistochemistry (IHC)	IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

28218-1-AP targets FSTL3/FLRG in WB, IHC, ELISA applications and shows reactivity with human, rat samples.

Tested Reactivity	human, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag27626 Product name: Recombinant human FSTL3 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 94-263 aa of BC005839 Sequence: PCKDSCDGVECGPGKACRMLGGRPRCECAPDCSGLPARLQVCGSDGATYRDECELRAARCRGHPDLSVMYRGRCRKSCEHVVCPRPQSCVVDQTGSAHCVVCRAAPCPVPSSPGQELCGNNNVTYISSCHMRQATCFLGRSIGVRHAGSCAGTPEEPPGGESAEEEENFV Predict reactive species
Full Name	follistatin-like 3 (secreted glycoprotein)
Calculated Molecular Weight	263 aa, 28 kDa
Observed Molecular Weight	28-33 kDa
GenBank Accession Number	BC005839
Gene Symbol	FSTL3
Gene ID (NCBI)	10272
RRID	AB_3086037
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	O95633
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Follistatin‐like 3 (FSTL3) is a highly conserved 27-39 kDa monomeric glycoprotein belonging to the follistatin protein family and it is primarily expressed in the placenta as well as in the testis, heart, adipose tissue, and pancreas. It is an extracellular inhibitor of the transforming growth factor beta (TGF‐β) family ligands, including activin, myostatin, and bone morphogenetic proteins.