Tested Applications
| Positive WB detected in | mouse liver tissue, HepG2 cells, rat liver tissue |
| Positive IHC detected in | human liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:4000-1:16000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21959-1-AP targets FTCD in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag16646 Product name: Recombinant human FTCD protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 192-541 aa of BC136395 Sequence: TKEQAHRIALNLREQGRGKDQPGRLKKVQGIGWYLDEKNLAQVSTNLLDFEVTALHTVYEETCREAQELSLPVVGSQLVGLVPLKALLDAAAFYCEKENLFILEEEQRIRLVVSRLGLDSLCPFSPKERIIEYLVPERGPERGLGSKSLRAFVGEVGARSAAPGGGSVAAAAAAMGAALGSMVGLMTYGRRQFQSLDTTMRRLIPPFREASAKLTTLVDADAEAFTAYLEAMRLPKNTPEEKDRRTAALQEGLRRAVSVPLTLAETVASLWPALQELARCGNLACRSDLQVAAKALEMGVFGAYFNVLINLRDITDEAFKDQIHHRVSSLLQEAKTQAALVLDCLETRQE Predict reactive species |
| Full Name | formiminotransferase cyclodeaminase |
| Calculated Molecular Weight | 541 aa, 59 kDa |
| Observed Molecular Weight | 53-62 kDa |
| GenBank Accession Number | BC136395 |
| Gene Symbol | FTCD |
| Gene ID (NCBI) | 10841 |
| RRID | AB_11182396 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | O95954 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FTCD (formimidoyltransferase cyclodeaminase) is critical for the catabolism of histidine. This process is one-carbon units that can enter the one-carbon/folate cycle, which provides methyl groups for arsenic metabolism (PMID: 30893314). In addition, FTCD could serve as a predictive and prognostic marker for patients with HCC (PMID: 37675273).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FTCD antibody 21959-1-AP | Download protocol |
| WB protocol for FTCD antibody 21959-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FTCD Polyclonal antibody (Cat# 21959-1-AP) has 6 citations published in Frontiers in Cell and Developmental Biology, Scientific Reports, iScience, FASEB Journal, Medical Science Monitor, and Bioengineered. Research fields span hepatocellular carcinoma (liver zonation, metabolic reprogramming, non-apoptotic cell death, iron metabolism, and prognostic markers), hepatic one-carbon metabolism in homocystinuria, and FTCD's role in apoptosis, DNA damage, and PI3K/Akt signaling. Applications cited include WB, IHC, and IF in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Sci Rep Quantitative proteomics by SWATH-MS reveals sophisticated metabolic reprogramming in hepatocellular carcinoma tissues. | ||
iScience Crosstalk of non-apoptotic RCD panel in hepatocellular carcinoma reveals the prognostic and therapeutic optimization | ||
FASEB J Dysregulation of hepatic one-carbon metabolism in classical homocystinuria: Implications of redox-sensitive DHFR repression and tetrahydrofolate depletion for pathogenesis and treatment | ||
Med Sci Monit Formiminotransferase Cyclodeaminase Suppresses Hepatocellular Carcinoma by Modulating Cell Apoptosis, DNA Damage, and Phosphatidylinositol 3-Kinases (PI3K)/Akt Signaling Pathway. | ||
Bioengineered A novel four-gene of iron metabolism-related and methylated for prognosis prediction of hepatocellular carcinoma. |
Reviews
What customers say
The product has one review, rated 5 stars. The reviewer used it for Western Blot at a 1:4000 dilution on house liver and HepG2 cells, describing it as a "very good antibody." Overall, the feedback is positive, indicating reliable performance in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Hua (Verified Customer) (05-11-2022) | Very good antibody
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