Tested Applications
| Positive WB detected in | A549 cells, HSC-T6 cells, K-562 cells, rabbit brain tissue, U-251 cells, HeLa cells, C6 cells, rat heart tissue, rat liver tissue, rat brain tissue, rabbit heart tissue, PC-3 cells, HaCaT cells |
| Positive IHC detected in | rat liver tissue, mouse liver tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HepG2 cells, HeLa cells, C6 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
60875-5-Ig targets Ferritin heavy chain in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, rat, rabbit samples.
| Tested Reactivity | human, rat, rabbit |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Mouse / IgG2a |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag21386 Product name: Recombinant human FTH1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-183 aa of BC000857 Sequence: MTTASTSQVRQNYHQDSEAAINRQINLELYASYVYLSMSYYFDRDDVALKNFAKYFLHQSHEEREHAEKLMKLQNQRGGRIFLQDIKKPDCDDWESGLNAMECALHLEKNVNQSLLELHKLATDKNDPHLCDFIETHYLNEQVKAIKELGDHVTNLRKMGAPESGLAEYLFDKHTLGDSDNES Predict reactive species |
| Full Name | ferritin, heavy polypeptide 1 |
| Calculated Molecular Weight | 183 aa, 21 kDa |
| Observed Molecular Weight | 21 kDa |
| GenBank Accession Number | BC000857 |
| Gene Symbol | FTH1 |
| Gene ID (NCBI) | 2495 |
| RRID | AB_3743235 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein A purification |
| UNIPROT ID | P02794 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Ferritin heavy chain(FTH1, ferritin, heavy polypeptide 1 ), as a 21 kDa subunit of the ferritin complex, exhibits ferroxidase activity, which serves an essential role in catalyzing the conversion of the ferrous ions (Fe2+) to the ferric form (Fe3+). FTH1 regulates angiogenesis during inflammation and malignancy by binding to high molecular weight kininogen (HKa) and blocking its anti-angiogenic activity on endothelial cells in vitro and in vivo(PMID: 33260500). FTH1 has been shown to protect proximal tube epithelial cells and kidneys against the activity of free iron in reactive oxygen species generation. FTH1 plays an important role in the maintenance of the cellular iron balance in ferroptosis.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Ferritin heavy chain antibody 60875-5-Ig | Download protocol |
| IHC protocol for Ferritin heavy chain antibody 60875-5-Ig | Download protocol |
| WB protocol for Ferritin heavy chain antibody 60875-5-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Ferritin Heavy Chain (FTH1) Monoclonal Antibody (3G8D7), catalog number 60875-5-Ig, has 12 citations. These appear in journals including Oncogene, Free Radical Biology and Medicine, ACS Applied Materials & Interfaces, Environ International, International Journal of Surgery, International Immunopharmacology, Journal of Molecular Medicine, Scientific Reports, Molecular Carcinogenesis, Neurological Research, and Redox Biology. Research fields span ferroptosis, ferritinophagy, and iron homeostasis in cancers (lung, breast, gastric, hepatocellular), osteoporosis, colitis, sepsis, cerebral ischemia, and nerve regeneration.
| Species | Application | Title |
|---|---|---|
Environ Int Environmental heat exposure triggers neuronal apoptosis via NCOA4-mediated ferritinophagy. | ||
Oncogene SRD5A3-mediated aberrant N-glycosylation of SCARA5 promotes ferroptosis in lung adenocarcinoma. | ||
Int J Surg Targeting SIRT3 to regulate mitophagy-dependent ferroptosis for preventing glucocorticoid-induced osteoporosis | ||
Free Radic Biol Med Difenoconazole impairs male reproductive function by inducing spermatogenic ferroptosis via the Wnt/β-catenin/NCOA4-mediated ferritinophagy axis. | ||
Free Radic Biol Med Cancer cell membrane and iRGD peptide co-modified cantharidin liposomes for targeted therapy and immunotherapy of triple-negative breast cancer. | ||
ACS Appl Mater Interfaces Nanovesicles Synergistically Regulate Ferroptosis Characteristic Macrophage-Induced Angiogenesis via Ferritin Heavy Chain 1/Glutathione Peroxidase 4 Pathway to Promote Peripheral Nerve Regeneration. |
Reviews
What customers say
The single review for this antibody reports a 5-star rating from a Western Blot application on an Ewing sarcoma cell line, using a 1:10,000 dilution. The reviewer described it as a very good antibody that can be diluted further, indicating strong performance and favorable cost-efficiency. Overall, the feedback is positive, highlighting reliable results and the ability to use higher dilutions without compromising signal quality.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Nenggang (Verified Customer) (08-17-2026) | Very good antibody, can be dilute further.
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