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Ferritin light chain Polyclonal antibody
Ferritin light chain Polyclonal Antibody for IF, IHC, IP, WB,ELISA
Cat no : 10727-1-AP
Validation Data Gallery
|Positive WB detected in||mouse liver tissue, human placenta tissue, mouse ovary tissue, mouse brain tissue, mouse spleen tissue|
|Positive IP detected in||mouse liver tissue|
|Positive IHC detected in||human liver cancer tissue, human liver tissue, human spleen tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Positive IF detected in||HepG2 cells|
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:2000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:100-1:500|
|Immunofluorescence (IF)||IF : 1:10-1:100|
|Sample-dependent, check data in validation data gallery|
10727-1-AP targets Ferritin light chain in WB, IP, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||human, monkey, mouse, rat|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Ferritin light chain fusion protein Ag1149|
|Full Name||ferritin, light polypeptide|
|Calculated molecular weight||19 kDa|
|Observed molecular weight||20-22 kDa|
|GenBank accession number||BC008439|
|Gene ID (NCBI)||2512|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
FTL belongs to the ferritin family. It stores iron in a soluble, non-toxic, readily available form. FTL is important for iron homeostasis. It plays a role in delivery of iron to cells. FTL mediates iron uptake in capsule cells of the developing kidney. Mutation of FTL will cause hereditary hyperferritinemia-cataract syndrome (HHCS) or neurodegeneration with brain iron accumulation type 3 (NBIA3). Ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH1) were the main constituents the striatum and cerebellar cortex revealed.
|Product Specific Protocols|
|WB protocol for Ferritin light chain antibody 10727-1-AP||Download protocol|
|IHC protocol for Ferritin light chain antibody 10727-1-AP||Download protocol|
|IF protocol for Ferritin light chain antibody 10727-1-AP||Download protocol|
|IP protocol for Ferritin light chain antibody 10727-1-AP||Download protocol|
|Click here to view our Standard Protocols|
Aging (Albany NY)
Tissue iron is negatively correlated with TERC or TERT mRNA expression: A heterochronic parabiosis study in mice.
Cell Death Dis
Pyroptosis and ferroptosis induced by mixed lineage kinase 3 (MLK3) signaling in cardiomyocytes are essential for myocardial fibrosis in response to pressure overload.
Nuclear transcription factor Nrf2 suppresses prostate cancer cells growth and migration through upregulating ferroportin.
Hepcidin attenuates the iron-mediated secondary neuronal injury after intracerebral hemorrhage in rats.
Neuroimaging, Urinary, and Plasma Biomarkers of Treatment Response in Huntington's Disease: Preclinical Evidence with the p75NTR Ligand LM11A-31.
Oxid Med Cell Longev
Ferroportin-Dependent Iron Homeostasis Protects against Oxidative Stress-Induced Nucleus Pulposus Cell Ferroptosis and Ameliorates Intervertebral Disc Degeneration In Vivo.