Tested Applications
| Positive WB detected in | mouse liver tissue, human placenta tissue, mouse ovary tissue, mouse testis tissue, mouse spleen tissue, rat liver tissue |
| Positive IP detected in | mouse liver tissue |
| Positive IHC detected in | human liver tissue, human liver cancer tissue, mouse spleen tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | A549 cells |
This antibody is not recommended for WB detection of brain sample.
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:10000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10727-1-AP targets Ferritin light chain in WB, IHC, IF/ICC, IP, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat, monkey, bovine, hamster, duck |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1149 Product name: Recombinant human FTL protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-175 aa of BC008439 Sequence: MSSQIRQNYSTDVEAAVNSLVNLYLQASYTYLSLGFYFDRDDVALEGVSHFFRELAEEKREGYERLLKMQNQRGGRALFQDIKKPAEDEWGKTPDAMKAAMALEKKLNQALLDLHALGSARTDPHLCDFLETHFLDEEVKLIKKMGDHLTNLHRLGGPEAGLGEYLFERLTLKHD Predict reactive species |
| Full Name | ferritin, light polypeptide |
| Calculated Molecular Weight | 19 kDa |
| Observed Molecular Weight | 18-20 kDa |
| GenBank Accession Number | BC008439 |
| Gene Symbol | Ferritin light chain |
| Gene ID (NCBI) | 2512 |
| RRID | AB_2278673 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P02792 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FTL belongs to the ferritin family. It stores iron in a soluble, non-toxic, readily available form. FTL is important for iron homeostasis. It plays a role in delivery of iron to cells. FTL mediates iron uptake in capsule cells of the developing kidney. Mutation of FTL will cause hereditary hyperferritinemia-cataract syndrome (HHCS) or neurodegeneration with brain iron accumulation type 3 (NBIA3). Ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH1) were the main constituents the striatum and cerebellar cortex revealed.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Ferritin light chain antibody 10727-1-AP | Download protocol |
| IHC protocol for Ferritin light chain antibody 10727-1-AP | Download protocol |
| IP protocol for Ferritin light chain antibody 10727-1-AP | Download protocol |
| WB protocol for Ferritin light chain antibody 10727-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Adipocytes orchestrate obesity-related chronic inflammation through β2-microglobulin | ||
Cell Metab Reactive oxygen species regulation by NCF1 governs ferroptosis susceptibility of Kupffer cells to MASH | ||
Mil Med Res Caveolin-1 is critical for hepatic iron storage capacity in the development of nonalcoholic fatty liver disease |
Reviews
What customers say
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Zakia (Verified Customer) (08-21-2026) | Demonstrates good lane-to-lane consistency across samples, successfully marking the target subunit region between 17 and 28 kDa.The bands in this specific image appear somewhat diffuse, but this is entirely a technical artifact of the membrane transfer stage (common with small ~20 kDa proteins) and not a reflection of antibody quality. satisfied with this antibody.
 |
Laura (Verified Customer) (08-26-2024) | used for WB
|
Amandine (Verified Customer) (02-07-2024) | 25ug of protein DENATURED, PVDF membrane, primary antibody 1/10000 1h30 RT, secondary antibody 1/5000 2h RT. Chemiluminescence revelation, 6min.
 |
