FTL Recombinant monoclonal antibody, PBS Only (Capture)

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• FTL Recombinant monoclonal antibody, PBS Only (Capture) (81166-5-PBS)

FTL Uni-rAb^® Recombinant Antibody for Sandwich ELISA, Indirect ELISA

Cat No. 81166-5-PBS

Clone No.251048G8

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

Sandwich ELISA, Indirect ELISA

Synonyms

Ferritin, Ferritin light chain, Ferritin L subunit

• 

  Sandwich ELISA sample test of MP02505-1

  Urine of six individual healthy human donors was measured. The human Ferritin Light Chain concentration of detected samples was determined to be 34.7 pg/mL with a range of ND-54.7 pg/mL.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Sandwich ELISA sample test of MP02505-1

  Saliva of six individual healthy human donors was measured. The human Ferritin Light Chain concentration of detected samples was determined to be 0.13 ng/mL with a range of 0.03-0.21 ng/mL.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Sandwich ELISA sample test of MP02505-1

  Plasma of six individual healthy human donors was measured. The human Ferritin Light Chain concentration of detected samples was determined to be 6.80 ng/mL with a range of 0.51-35.68 ng/mL.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Sandwich ELISA sample test of MP02505-1

  Human peripheral blood mononuclear cells (PBMC) were cultured unstimulated for 3 days. The mean Ferritin Light Chain concentration was determined to be 0.21 ng/mL in cell culture supernatant.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Sandwich ELISA sample test of MP02505-1

  The mean Ferritin Light Chain concentration was determined to be 18.93 ng/mL in HeLa cell extract based on a 1.2 mg/mL extract load.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Sandwich ELISA sample test of MP02505-1

  Human milk of six individual healthy human donors was measured. The human Ferritin Light Chain concentration of detected samples was determined to be 2.12 ng/mL with a range of 0.15-10.14 ng/mL.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  Sandwich ELISA standard curve of MP02505-1

  Sandwich ELISA standard curve of MP02505-1, Human Ferritin Light Chain Recombinant Matched Antibody Pair - PBS only. 81166-5-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag20637. 81166-4-PBS was HRP conjugated as the detection antibody. Range: 0.156-10 ng/mL

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Product Information

81166-5-PBS targets FTL as part of a matched antibody pair:

MP02505-1: 81166-5-PBS capture and 81166-4-PBS detection (validated in Sandwich ELISA)

Unconjugated rabbit recombinant monoclonal antibody in PBS only (BSA and azide free) storage buffer at a concentration of 1 mg/mL, ready for conjugation. Created using Proteintech’s proprietary in-house recombinant technology. Recombinant production enables unrivalled batch-to-batch consistency, easy scale-up, and future security of supply.

This conjugation ready format makes antibodies ideal for use in many applications including: ELISAs, multiplex assays requiring matched pairs, mass cytometry, and multiplex imaging applications.Antibody use should be optimized by the end user for each application and assay.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag20637

  Product name: Recombinant human FTL protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-175 aa of BC008439

  Sequence: MSSQIRQNYSTDVEAAVNSLVNLYLQASYTYLSLGFYFDRDDVALEGVSHFFRELAEEKREGYERLLKMQNQRGGRALFQDIKKPAEDEWGKTPDAMKAAMALEKKLNQALLDLHALGSARTDPHLCDFLETHFLDEEVKLIKKMGDHLTNLHRLGGPEAGLGEYLFERLTLKHD

• Full Name: ferritin, light polypeptide
• Calculated Molecular Weight: 19 kDa
• GenBank Accession Number: BC008439
• Gene Symbol: Ferritin light chain
• Gene ID (NCBI): 2512
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P02792
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.