FUBP3 Polyclonal antibody

FUBP3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Cat no : 10623-1-AP

Synonyms

FBP3, FUBP3, FUSE binding protein 3

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Validation Data Gallery

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  WB analysis of HEK-293 using 10623-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10623-1-AP (FUBP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10623-1-AP

  IP result of anti-FUBP3 (IP:10623-1-AP, 4ug; Detection:10623-1-AP 1:500) with HEK-293 cells lysate 1800ug.

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  IHC staining of human prostate cancer using 10623-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10623-1-AP (FUBP3 antibody) at dilution of 1:100 (under 25x lens).

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 1 publications below

Product Information

10623-1-AP targets FUBP3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FUBP3 fusion protein Ag0974
• Full Name: far upstream element (FUSE) binding protein 3
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC007874
• Gene Symbol: FUBP3
• Gene ID (NCBI): 8939
• RRID: AB_2104940
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FUBP3, also named as Far upstream element-binding protein 3, is a 572 amino acid protein, which is detected in a number of cell lines and localizes in the nucleus. FUBP3 may interact with single-stranded DNA from the far-upstream element (FUSE) and activate gene expression. FUBP3 exists some isoforms with MV 62 kDa and 28 kDa.

Publications

Species	Application	Title
human	WB,IHC	Oncogene; Long noncoding RNA CMPK2 promotes colorectal cancer progression by activating the FUBP3-c-Myc axis.; Authors - Qingzu Gao
human	WB,IF	J Immunol; REDD1/Autophagy Pathway Is Associated with Neutrophil-Driven IL-1β Inflammatory Response in Active Ulcerative Colitis.; Authors - Iliana Angelidou
human	WB,IF,IP	J Virol; FUBP3 Degrades the Porcine Epidemic Diarrhea Virus Nucleocapsid Protein and Induces the Production of Type I Interferon.; Authors - Sujie Dong
human,pig	WB	Autophagy; N protein of PEDV plays chess game with host proteins by selective autophagy; Authors - Xueying Zhai
human	WB	Antiviral Res; Proteomic and phosphoproteomic analysis of responses to enterovirus A71 infection reveals novel targets for antiviral and viral replication; Authors - Dandan Lin