FUBP3 Polyclonal antibody

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• FUBP3 Polyclonal antibody (10623-1-AP)

FUBP3 Polyclonal Antibody for WB, IP, IHC, ELISA

Cat No. 10623-1-AP

Publications(9)

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF, IP, ELISA

Synonyms

FBP3, FUBP3, FUSE binding protein 3

• 

  WB analysis of HEK-293 using 10623-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10623-1-AP (FUBP3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HEK-293 using 10623-1-AP

  IP result of anti-FUBP3 (IP:10623-1-AP, 4ug; Detection:10623-1-AP 1:500) with HEK-293 cells lysate 1800ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human prostate cancer using 10623-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10623-1-AP (FUBP3 antibody) at dilution of 1:100 (under 25x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells
• Positive IP detected in: HEK-293 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

10623-1-AP targets FUBP3 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, pig
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag0974

  Product name: Recombinant human FUBP3 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-261 aa of BC007874

  Sequence: MPPIGNQLGALVHQRTVITEEFKVPDKMVGFIIGRGGEQISRIQAESGCKIQIASESSGIPERPCVLTGTPESIEQAKRLLGQIVDRCRNGPGFHNDIDSNSTIQEILIPASKVGLVIGRGGETIKQLQERTGVKMVMIQDGPLPTGADKPLRITGDAFKVQQAREMVLEIIREKDQADFRGVRGDFNSRMGGGSIEVSVPRFAVGIVIGRNGEMIKKIQNDAGVRIQFKPDDGISPEYYRQQVAFYGQTLGQAQAHSQEQ

• Full Name: far upstream element (FUSE) binding protein 3
• Calculated Molecular Weight: 28 kDa
• Observed Molecular Weight: 62 kDa
• GenBank Accession Number: BC007874
• Gene Symbol: FUBP3
• Gene ID (NCBI): 8939
• RRID: AB_2104940
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96I24
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FUBP3, also named as Far upstream element-binding protein 3, is a 572 amino acid protein, which is detected in a number of cell lines and localizes in the nucleus. FUBP3 may interact with single-stranded DNA from the far-upstream element (FUSE) and activate gene expression. FUBP3 exists some isoforms with MV 62 kDa and 28 kDa.

Publications

What published studies show

Automatically generated from the publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC	Oncogene; Long noncoding RNA CMPK2 promotes colorectal cancer progression by activating the FUBP3-c-Myc axis.; Authors - Qingzu Gao
human	WB,IF	J Immunol; REDD1/Autophagy Pathway Is Associated with Neutrophil-Driven IL-1β Inflammatory Response in Active Ulcerative Colitis.; Authors - Iliana Angelidou
human	WB,IF,IP	J Virol; FUBP3 Degrades the Porcine Epidemic Diarrhea Virus Nucleocapsid Protein and Induces the Production of Type I Interferon.; Authors - Sujie Dong
human		Mol Cell; TurboCas: A method for locus-specific labeling of genomic regions and isolating their associated protein interactome; Authors - Bercin K Cenik ; KD Validated
	WB	Virology; The UP1 domain is essential for the facilitation effect of HnRNP A1 on PRRSV-2 replication; Authors - Huiyuan Jing
human	WB	Antiviral Res; Proteomic and phosphoproteomic analysis of responses to enterovirus A71 infection reveals novel targets for antiviral and viral replication; Authors - Dandan Lin