FUBP3 Polyclonal antibody

FUBP3 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IF, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10623-1-AP

Synonyms

FBP3, FUBP3, FUSE binding protein 3



Tested Applications

Positive WB detected inHEK-293 cells
Positive IP detected inHEK-293 cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10623-1-AP targets FUBP3 in WB, IP, IF, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FUBP3 fusion protein Ag0974
Full Name far upstream element (FUSE) binding protein 3
Calculated Molecular Weight 28 kDa
Observed Molecular Weight 62 kDa
GenBank Accession NumberBC007874
Gene Symbol FUBP3
Gene ID (NCBI) 8939
RRIDAB_2104940
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FUBP3, also named as Far upstream element-binding protein 3, is a 572 amino acid protein, which is detected in a number of cell lines and localizes in the nucleus. FUBP3 may interact with single-stranded DNA from the far-upstream element (FUSE) and activate gene expression. FUBP3 exists some isoforms with MV 62 kDa and 28 kDa.

Protocols

Product Specific Protocols
WB protocol for FUBP3 antibody 10623-1-APDownload protocol
IHC protocol for FUBP3 antibody 10623-1-APDownload protocol
IP protocol for FUBP3 antibody 10623-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB,IHC

Oncogene

Long noncoding RNA CMPK2 promotes colorectal cancer progression by activating the FUBP3-c-Myc axis.

Authors - Qingzu Gao
humanWB,IF

J Immunol

REDD1/Autophagy Pathway Is Associated with Neutrophil-Driven IL-1β Inflammatory Response in Active Ulcerative Colitis.

Authors - Iliana Angelidou
humanWB,IF,IP

J Virol

FUBP3 Degrades the Porcine Epidemic Diarrhea Virus Nucleocapsid Protein and Induces the Production of Type I Interferon.

Authors - Sujie Dong
human,pigWB

Autophagy

N protein of PEDV plays chess game with host proteins by selective autophagy

Authors - Xueying Zhai
humanWB

Antiviral Res

Proteomic and phosphoproteomic analysis of responses to enterovirus A71 infection reveals novel targets for antiviral and viral replication

Authors - Dandan Lin