FUCA2 Polyclonal antibody

FUCA2 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 15157-1-AP

Synonyms

Alpha L fucosidase 2, FUCA2, fucosidase, alpha L 2, plasma, Plasma alpha L fucosidase, UNQ227/PRO260

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Validation Data Gallery

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  WB analysis of human heart using 15157-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15157-1-AP (FUCA2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 15157-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15157-1-AP (FUCA2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 15157-1-AP

  IP result of anti-FUCA2 (IP:15157-1-AP, 4ug; Detection:15157-1-AP 1:300) with mouse heart tissue lysate 4000 ug.

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  IHC staining of human liver using 15157-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15157-1-AP (FUCA2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 15157-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15157-1-AP (FUCA2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human hepatocirrhosis using 15157-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15157-1-AP (FUCA2 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 15157-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis tissue slide using 15157-1-AP (FUCA2 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 15157-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15157-1-AP (FUCA2 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 15157-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15157-1-AP (FUCA2 Antibody) at dilution of 1:50 (under 40x lens).

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Tested Applications

• Positive WB detected in: human heart tissue, mouse stomach tissue, rat stomach tissue
• Positive IP detected in: mouse heart tissue
• Positive IHC detected in: human liver tissue, human hepatocirrhosis tissue, human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

15157-1-AP targets FUCA2 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FUCA2 fusion protein Ag7250
• Full Name: fucosidase, alpha-L- 2, plasma
• Calculated Molecular Weight: 54 kDa
• Observed Molecular Weight: 54-60 kDa
• GenBank Accession Number: BC003060
• Gene Symbol: FUCA2
• Gene ID (NCBI): 2519
• RRID: AB_2104956
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Alpha-L-fucosidase 2 (FUCA2) is a gene that codes for a secreted non-lysosomal enzyme and belongs to the glycosyl hydrolase 29 (GH29) family. The enzyme is responsible for the removal of the alpha-1,6-fucose linked to N-acetylglucosamine residue of glycoproteins. Serum FUCA2 is a potential biomarker used for early detection of esophageal squamous cell carcinoma (ESCC) and hepatocellular carcinoma (HCC). FUCA2 has 2 isoforms with the molecular mass of 20 and 54 kDa (PMID: 34935631). Sometimes higher molecular weight around 60 kDa can also be observed, which may be a modified variant of FUCA2.

Publications

Species	Application	Title
human,mouse	WB	Nat Commun; Proteomic screens of SEL1L-HRD1 ER-associated degradation substrates reveal its role in glycosylphosphatidylinositol-anchored protein biogenesis; Authors - Xiaoqiong Wei

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Gretchen (Verified Customer) (02-01-2019)

I did a 1:2000 dilution in 30ug of lysate protein. The bands were sharp and strong and it took 3 seconds to develop. However, I had a lot of unspecific bands and not only at 86, they were some stronger than others.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Melanoma cells