|Positive WB detected in||HeLa cells, COLO 320 cells|
|Positive IP detected in||HeLa cells|
|Positive IHC detected in||human placenta tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunoprecipitation (IP)||IP : 0.5-4.0 ug for IP and 1:500-1:1000 for WB|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
The immunogen of 18413-1-AP is FURIN Fusion Protein expressed in E. coli.
|Cited Reactivity||chicken, human|
|Host / Isotype||Rabbit / IgG|
|Immunogen||FURIN fusion protein Ag13261|
|Full Name||furin (paired basic amino acid cleaving enzyme)|
|Calculated molecular weight||87 kDa|
|Observed molecular weight||90-110 kDa|
|GenBank accession number||BC012181|
|Gene ID (NCBI)||5045|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
FURIN, also named as FUR, PACE, PCSK3, SPC1, Kex2p, is likely to represent the ubiquitous endoprotease activity within constitutive secretory pathways and capable of cleavage at the RX(K/R)R consensus motif. Furin is synthesized as an inactive zymogen that may minimize the occurrence of premature enzymatic activity that would lead to alternative protein activation or degradation. Glycosylation and other post-translational modifications can make the pre-pro furin run at 100-110 kDa, the mature furin at 98-95 kDa and shed furin at 90 kDa.
Synergistic antitumor effects of 9.2.27-PE38KDEL and ABT-737 in primary and metastatic brain tumors.
Furin inhibitor D6R suppresses epithelial-mesenchymal transition in SW1990 and PaTu8988 cells via the Hippo-YAP signaling pathway.
Cellular CARD11 Inhibits the Fusogenic Activity of Newcastle Disease Virus via CBM Signalosome-Mediated Furin Reduction in Chicken Fibroblasts.
SNAI1, an endothelial-mesenchymal transition transcription factor, promotes the early phase of ocular neovascularization.
Br J Cancer
Lipoic acid decreases breast cancer cell proliferation by inhibiting IGF-1R via furin downregulation.
Methyl-CpG-binding protein 2 drives the Furin/TGF-β1/Smad axis to promote epithelial-mesenchymal transition in pancreatic cancer cells.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Boyan (Verified Customer) (03-11-2019)
For WB, the expected band was strong; for IF, PFA fixation, it could label the cell surface-like localisation and the Golgi localisation of Furin.
Alex (Verified Customer) (10-23-2018)
Cells were fixed with 4% PFA, permeabilized with saponin, and stained with anti-furin.