FUSIP1/SRS10 Polyclonal antibody

• Featured Product
• KD/KO Validated

FUSIP1/SRS10 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

3

Cat no : 10131-1-AP

Synonyms

FUSIP1, Serine/arginine-rich splicing factor 10, NSSR, FUSIP2, FUS-interacting serine-arginine-rich protein 1

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Validation Data Gallery

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  WB analysis of HepG2 using 10131-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10131-1-AP (FUSIP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 10131-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10131-1-AP (FUSIP1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 10131-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10131-1-AP (FUSIP1 Antibody) at dilution of 1:300 incubated at 4 degree celsius over night.

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  IHC staining of human urothelial carcinoma using 10131-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10131-1-AP (FUSIP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 10131-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 10131-1-AP (FUSIP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: HepG2 cells, HeLa cells
• Positive IHC detected in: human urothelial carcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IHC: See 1 publications below

Product Information

10131-1-AP targets FUSIP1/SRS10 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FUSIP1/SRS10 fusion protein Ag0176
• Full Name: FUS interacting protein (serine/arginine-rich) 1
• Calculated Molecular Weight: 22 kDa
• Observed Molecular Weight: 31 kDa
• GenBank Accession Number: BC001107
• Gene Symbol: FUSIP1
• Gene ID (NCBI): 10772
• RRID: AB_2877726
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FUSIP1, also named as SRS10, or TASR, is a 262 amino acid protein, which belongs to the splicing factor SR family. FUSIP1 as a Splicing factor that in its dephosphorylated form acts as a general repressor of pre-mRNA splicing. FUSIP1 is required for splicing repression in M-phase cells and after heat shock and also acts as a splicing factor that specifically promotes exon skipping during alternative splicing. FUSIP1 Interacting with YTHDC1, a RNA-binding protein that recognizes and binds N6-methyladenosine (m6A)-containing RNAs, prevents SRSF10 from binding to its mRNA-binding sites close to m6A-containing regions, leading to inhibit exon skipping during alternative splicing.

Publications

Species	Application	Title
human	WB	Nat Commun; Mutually exclusive acetylation and ubiquitylation of the splicing factor SRSF5 control tumor growth.; Authors - Yuhan Chen
human	WB	J Exp Clin Cancer Res; SRSF10 inhibits biogenesis of circ-ATXN1 to regulate glioma angiogenesis via miR-526b-3p/MMP2 pathway.; Authors - Xiaobai Liu ; KD Validated
human	WB,IHC	Proteomics Clin Appl; Integration of the cancer cell secretome and transcriptome reveals potential noninvasive diagnostic markers for bladder cancer; Authors - Yi-Ting Chen