FUT3 Monoclonal antibody, PBS Only

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  WB analysis of human placenta using 67344-1-Ig (same clone as 67344-1-PBS)

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67344-1-Ig (FUT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67344-1-PBS in a different storage buffer formulation.

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  WB analysis of NCCIT using 67344-1-Ig (same clone as 67344-1-PBS)

  NCCIT cells were subjected to SDS PAGE followed by western blot with 67344-1-Ig (FUT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67344-1-PBS in a different storage buffer formulation.

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  WB analysis of JAR using 67344-1-Ig (same clone as 67344-1-PBS)

  JAR cells were subjected to SDS PAGE followed by western blot with 67344-1-Ig (FUT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67344-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 67344-1-Ig (same clone as 67344-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67344-1-Ig (FUT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67344-1-PBS in a different storage buffer formulation.

• 

  WB analysis of JAR using 67344-1-Ig (same clone as 67344-1-PBS)

  JAR cells were subjected to SDS PAGE followed by western blot with 67344-1-Ig (FUT3 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67344-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67344-1-Ig (same clone as 67344-1-PBS)

  Immunohistochemical analysis of paraffin-embedded colon cancer slide using 67344-1-Ig (FUT3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67344-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67344-1-Ig (same clone as 67344-1-PBS)

  Immunohistochemical analysis of paraffin-embedded colon cancer slide using 67344-1-Ig (FUT3 antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67344-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate hyperplasia using 67344-1-Ig (same clone as 67344-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 67344-1-Ig (FUT3 antibody) at dilution of 1:500 (under 10x lens).. This data was developed using the same antibody clone with 67344-1-PBS in a different storage buffer formulation.

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  IHC staining of human prostate hyperplasia using 67344-1-Ig (same clone as 67344-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate hyperplasia tissue slide using 67344-1-Ig (FUT3 antibody) at dilution of 1:500 (under 40x lens).. This data was developed using the same antibody clone with 67344-1-PBS in a different storage buffer formulation.

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  IF Staining of human prostate hyperplasia using 67344-1-Ig (same clone as 67344-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human prostate hyperplasia tissue using FUT3 antibody (67344-1-Ig, Clone: 2A6G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67344-1-PBS in a different storage buffer formulation.

• 

  IF Staining of human prostate hyperplasia using 67344-1-Ig (same clone as 67344-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed human prostate hyperplasia tissue using FUT3 antibody (67344-1-Ig, Clone: 2A6G7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67344-1-PBS in a different storage buffer formulation.

FUT3 Monoclonal Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 67344-1-PBS

Clone No.2A6G7

Host / Isotype

Mouse / IgG1

Reactivity

human

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

3-galactosyl-N-acetylglucosaminide 4-alpha-L-fucosyltransferase FUT3, 4-galactosyl-N-acetylglucosaminide 3-alpha-L-fucosyltransferase, Alpha-3-fucosyltransferase FUT3, Blood group Lewis alpha-4-fucosyltransferase, CD174

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Product Information

67344-1-PBS targets FUT3 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag12581

  Product name: Recombinant human FUT3 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 84-361 aa of BC108675

  Sequence: MVPGTADCHITADRKVYPQADTVIVHHWDIMSNPKSRLPPSPRPQGQRWIWFNLEPPPNCQHLEALDRYFNLTMSYRSDSDIFTPYGWLEPWSGQPAHPPLNLSAKTELVAWAVSNWKPDSARVRYYQSLQAHLKVDVYGRSHKPLPKGTMMETLSRYKFYLAFENSLHPDYITEKLWRNALEAWAVPVVLGPSRSNYERFLPPDAFIHVDDFQSPKDLARYLQELDKDHARYLSYFRWRETLRPRSFSWALDFCKACWKLQQESRYQTVRSIAAWFT

• Full Name: fucosyltransferase 3 (galactoside 3(4)-L-fucosyltransferase, Lewis blood group)
• Calculated Molecular Weight: 361 aa, 42 kDa
• Observed Molecular Weight: 42-45 kDa
• GenBank Accession Number: BC108675
• Gene Symbol: FUT3
• Gene ID (NCBI): 2525
• RRID: AB_2882602
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P21217
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.