Tested Applications
| Positive WB detected in | A549 cells |
| Positive IHC detected in | human breast cancer tissue, human tonsillitis tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:20-1:200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18197-1-AP targets FUT7 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12475 Product name: Recombinant human FUT7 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 1-342 aa of BC074746 Sequence: MNNAGHGPTRRLRGLGVLAGVALLAALWLLWLLGSAPRGTPAPQPTITILVWHWPFTDQPPELPSDTCTRYGIARCHLSANRSLLASADAVVFHHRELQTRRSHLPLAQRPRGQPWVWASMESPSHTHGLSHLRGIFNWVLSYRRDSDIFVPYGRLEPHWGPSPPLPAKSRVAAWVVSNFQERQLRARLYRQLAPHLRVDVFGRANGRPLCASCLVPTVAQYRFYLSFENSQHRDYITEKFWRNALVAGTVPVVLGPPRATYEAFVPADAFVHVDDFGSARELAAFLTGMNESRYQRFFAWRDRLRVRLFTDWRERFCAICDRYPHLPRSQVYEDLEGWFQA Predict reactive species |
| Full Name | fucosyltransferase 7 (alpha (1,3) fucosyltransferase) |
| Calculated Molecular Weight | 342 aa, 39 kDa |
| Observed Molecular Weight | 39-50 kDa |
| GenBank Accession Number | BC074746 |
| Gene Symbol | FUT7 |
| Gene ID (NCBI) | 2529 |
| RRID | AB_2878516 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q11130 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FUT7 antibody 18197-1-AP | Download protocol |
| IHC protocol for FUT7 antibody 18197-1-AP | Download protocol |
| WB protocol for FUT7 antibody 18197-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FUT7 Polyclonal antibody (Cat# 18197-1-AP) has 6 citations published in Cancer Cell, Advanced Science, International Journal of Molecular Medicine, Journal of Inflammation Research, Experimental Cell Research, and Evidence-Based Complementary and Alternative Medicine. Research fields span oncology (bladder carcinoma, thyroid carcinoma, leukemia, anti-tumor immunity), inflammatory bowel disease (Crohn's disease), reproductive biology (endometrial adhesion), and FUT7-mediated glycosylation signaling.
| Species | Application | Title |
|---|---|---|
Cancer Cell B cells disrupt tertiary lymphoid structure formation and suppress anti-tumor immunity. | ||
Adv Sci (Weinh) Western Diet Inhibits FUT7-Mediated Treg Intestinal Homing to Disrupt the Homeostasis of Intestinal Epithelial Cells in Crohn's Disease
| ||
Int J Mol Med miR‑519d‑3p released by human blastocysts negatively regulates endometrial epithelial cell adhesion by targeting HIF1α
| ||
J Inflamm Res FUT7 Promotes the Epithelial-Mesenchymal Transition and Immune Infiltration in Bladder Urothelial Carcinoma. | ||
Exp Cell Res FUT7 promotes the malignant transformation of follicular thyroid carcinoma through α1,3-Fucosylation of EGF receptor.
| ||
Evid Based Complement Alternat Med Fut7 Promotes Adhesion and Invasion of Acute Lymphoblastic Leukemia Cells through the Integrin/Fak/Akt Pathway. |
Reviews
What customers say
The single review for this product is negative. A researcher (Mark Esposito, Feb 2019) rated it 1 star after using it at 1:1000 dilution for Western Blot on human MDA-MB-231 cells overexpressing Fut7 (expression verified by qPCR). The antibody failed to detect the target under standard WB conditions. Overall, the limited feedback suggests reliability concerns for this application.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Mark (Verified Customer) (02-05-2019) | The antibody was used at 1:1000 in standard western blot conditions to detect ectopically expressed human fut7 that was verified by qPCR. Antibody did not work.
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