FUT8 Polyclonal antibody

• Featured Product
• KD/KO Validated

FUT8 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

4

Cat no : 12560-1-AP

Synonyms

alpha1 6FucT, Fucosyltransferase 8, FUT8

---

Validation Data Gallery

• 

  WB analysis of MCF-7 using 12560-1-AP

  WB result of FUT8 antibody (12560-1-AP; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FUT8 transfected MCF-7 cells.

• 

  IHC staining of human lung cancer using 12560-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12560-1-AP (FUT8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 12560-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer using 12560-1-AP (FUT8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human stomach cancer using 12560-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer slide using 12560-1-AP (FUT8 Antibody) at dilution of 1:50.

• 

  IHC staining of human stomach cancer using 12560-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer slide using 12560-1-AP (FUT8 Antibody) at dilution of 1:50.

---

Tested Applications

• Positive WB detected in: MCF-7 cells
• Positive IHC detected in: human lung cancer tissue, human stomach cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 3 publications below

Product Information

12560-1-AP targets FUT8 in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: FUT8 fusion protein Ag3280
• Full Name: fucosyltransferase 8 (alpha (1,6) fucosyltransferase)
• Calculated Molecular Weight: 66 kDa
• Observed Molecular Weight: 66-70 kDa
• GenBank Accession Number: BC025385
• Gene Symbol: FUT8
• Gene ID (NCBI): 2530
• RRID: AB_2294158
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fucosyltransferase 8 (FUT8) is GDP-fucose-glycoprotein fucosyltransferase and is localized in Golgi apparatus. FUT8 is involved in protein glycosylation by catalyzing the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans. FUT8 may be involved in human aging by regulating protein N-glycosylation and in the molecular pathogenesis of human pulmonary emphysema (PE).

Publications

Species	Application	Title
human		Am J Cancer Res; α1,6-Fucosyltransferase (FUT8) regulates the cancer-promoting capacity of cancer-associated fibroblasts (CAFs) by modifying EGFR core fucosylation (CF) in non-small cell lung cancer (NSCLC).; Authors - Fengzhou Li
human	IHC	Clin Exp Pharmacol Physiol; MicroRNA-198-5p inhibits the migration and invasion of non-small lung cancer cells by targeting fucosyltransferase 8.; Authors - Siyao Wang
human	IHC	Exp Mol Pathol; cDNA microarray analysis and immunohistochemistry reveal a distinct molecular phenotype in serous endometrial cancer compared to endometrioid endometrial cancer.; Authors - Chen Yonghua Y
human	WB,IHC	Biochem Biophys Rep; Partial silencing of fucosyltransferase 8 gene expression inhibits proliferation of Ishikawa cells, a cell line of endometrial cancer.; Authors - Hana Shimoyama ; KD Validated