Tested Applications
| Positive WB detected in | mouse brain tissue, NIH/3T3 cells, mouse cerebellum tissue, mouse kidney tissue |
| Positive IHC detected in | human stomach cancer tissue, human thyroid cancer tissue, human placenta tissue, human lung cancer tissue, human stomach cancer tissue, mouse cerebellum tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:4000 |
| Immunohistochemistry (IHC) | IHC : 1:300-1:1200 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
12560-1-AP targets FUT8 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3280 Product name: Recombinant human FUT8 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 276-575 aa of BC025385 Sequence: HWSGEVKDKNVQVVEFPIVDSLHPRPPYLPLAVPEDLADRLVRVHGDPAVWWVSQFVKYLIRPQPWLEKEIEEATKKLGFKHPVIGVHVRRTDKVGTEAAFHPIEEYMVHVEEHFQLLARRMQVDKKRVYLATDDPSLLKEAKTKYPNYEFISDNSISWSAGLHNRYTENSLRGVILDIHFLSQADFLVCTFSSQVCRVAYEIMQTLHPDASANFHSLDDIYYFGGQNAHNQIAIYAHQPRTADEIPMEPGDIIGVAGNHWDGYSKGVNRKLGRTGLYPSYKVREKIETVKYPTYPEAEK Predict reactive species |
| Full Name | fucosyltransferase 8 (alpha (1,6) fucosyltransferase) |
| Calculated Molecular Weight | 66 kDa |
| Observed Molecular Weight | 66-70 kDa |
| GenBank Accession Number | BC025385 |
| Gene Symbol | FUT8 |
| Gene ID (NCBI) | 2530 |
| RRID | AB_2294158 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9BYC5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Fucosyltransferase 8 (FUT8) is GDP-fucose-glycoprotein fucosyltransferase and is localized in Golgi apparatus. FUT8 is involved in protein glycosylation by catalyzing the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans. FUT8 may be involved in human aging by regulating protein N-glycosylation and in the molecular pathogenesis of human pulmonary emphysema (PE).
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for FUT8 antibody 12560-1-AP | Download protocol |
| WB protocol for FUT8 antibody 12560-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FUT8 Polyclonal antibody (Cat# 12560-1-AP) has 6 total citations. These appear in Science, Molecular Neurobiology, Experimental Molecular Pathology, Biochemical and Biophysical Reports, American Journal of Cancer Research, and Clinical and Experimental Pharmacology and Physiology. Research fields span neuronal autophagy and resilience, cerebellar neurogenesis, endometrial cancer molecular phenotyping, fucosyltransferase-mediated cancer proliferation, EGFR core fucosylation in non-small cell lung cancer, and microRNA-regulated lung cancer migration and invasion. Applications include WB and IHC in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Mol Neurobiol FUT8 Regulates Cerebellar Neurogenesis and Development Through Maintaining the Level of Neural Cell Adhesion Molecule Cntn2 | ||
Exp Mol Pathol cDNA microarray analysis and immunohistochemistry reveal a distinct molecular phenotype in serous endometrial cancer compared to endometrioid endometrial cancer. | ||
Biochem Biophys Rep Partial silencing of fucosyltransferase 8 gene expression inhibits proliferation of Ishikawa cells, a cell line of endometrial cancer.
| ||
Am J Cancer Res α1,6-Fucosyltransferase (FUT8) regulates the cancer-promoting capacity of cancer-associated fibroblasts (CAFs) by modifying EGFR core fucosylation (CF) in non-small cell lung cancer (NSCLC). | ||
Clin Exp Pharmacol Physiol MicroRNA-198-5p inhibits the migration and invasion of non-small lung cancer cells by targeting fucosyltransferase 8. |
