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FUT8 Polyclonal antibody

FUT8 Polyclonal Antibody for IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 12560-1-AP

Synonyms

alpha1 6FucT, Fucosyltransferase 8, FUT8



Tested Applications

Positive WB detected inMCF-7 cells
Positive IHC detected inhuman lung cancer tissue, human stomach cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:4000
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Product Information

12560-1-AP targets FUT8 in WB, IHC, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FUT8 fusion protein Ag3280
Full Name fucosyltransferase 8 (alpha (1,6) fucosyltransferase)
Calculated molecular weight 66 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC025385
Gene symbol FUT8
Gene ID (NCBI) 2530
RRIDAB_2294158
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fucosyltransferase 8 (FUT8) is GDP-fucose-glycoprotein fucosyltransferase and is localized in Golgi apparatus. FUT8 is involved in protein glycosylation by catalyzing the addition of fucose in alpha 1-6 linkage to the first GlcNAc residue, next to the peptide chains in N-glycans. FUT8 may be involved in human aging by regulating protein N-glycosylation and in the molecular pathogenesis of human pulmonary emphysema (PE).

Protocols

Product Specific Protocols
WB protocol for FUT8 antibody 12560-1-APDownload protocol
IHC protocol for FUT8 antibody 12560-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
human

Am J Cancer Res

α1,6-Fucosyltransferase (FUT8) regulates the cancer-promoting capacity of cancer-associated fibroblasts (CAFs) by modifying EGFR core fucosylation (CF) in non-small cell lung cancer (NSCLC).

Authors - Fengzhou Li
humanIHC

Clin Exp Pharmacol Physiol

MicroRNA-198-5p inhibits the migration and invasion of non-small lung cancer cells by targeting fucosyltransferase 8.

Authors - Siyao Wang
humanIHC

Exp Mol Pathol

cDNA microarray analysis and immunohistochemistry reveal a distinct molecular phenotype in serous endometrial cancer compared to endometrioid endometrial cancer.

Authors - Chen Yonghua Y
humanWB,IHC

Biochem Biophys Rep

Partial silencing of fucosyltransferase 8 gene expression inhibits proliferation of Ishikawa cells, a cell line of endometrial cancer.

Authors - Hana Shimoyama
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