Tested Applications
| Positive WB detected in | HEK-293 cells, mouse heart tissue, NIH/3T3 cells |
| Positive IP detected in | mouse skeletal muscle tissue |
| Positive IHC detected in | human placenta tissue, human heart tissue, mouse adrenal gland tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | HeLa cells, HepG2 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:1000-1:4000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
13194-1-AP targets FXR1 in WB, IHC, IF/ICC, IP, RIP, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human, mouse, rat, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag3873 Product name: Recombinant human FXR1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-353 aa of BC028983 Sequence: MAELTVEVRGSNGAFYKGFIKDVHEDSLTVVFENNWQPERQVPFNEVRLPPPPDIKKEISEGDEVEVYSRANDQEPCGWWLAKVRMMKGEFYVIEYAACDATYNEIVTFERLRPVNQNKTVKKNTFFKCTVDVPEDLREACANENAHKDFKKAVGACRIFYHPETTQLMILSASEATVKRVNILSDMHLRSIRTKLMLMSRNEEATKHLECTKQLAAAFHEEFVVREDLMGLAIGTHGSNIQQARKVPGVTAIELDEDTGTFRIYGESADAVKKARGFLEFVEDFIQVPRNLVGKVIGKNGKVIQEIVDKSGVVRVRIEGDNENKLPREDGMVPFVFVGTKESIGNVQVLLEY Predict reactive species |
| Full Name | fragile X mental retardation, autosomal homolog 1 |
| Calculated Molecular Weight | 621 aa, 70 kDa |
| Observed Molecular Weight | 70-80 kDa |
| GenBank Accession Number | BC028983 |
| Gene Symbol | FXR1 |
| Gene ID (NCBI) | 8087 |
| RRID | AB_2110702 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P51114 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Tumour necrosis factor-alpha (TNF-alpha) is a key mediator of inflammation in host defence against infection and in autoimmune disease. Its production is controlled post-transcriptionally by multiple RNA-binding proteins that interact with the TNF-alpha AU-rich element and regulate its expression; Fragile X mental retardation-related protein 1 (FXR1) is one of these. FXR1 (fragile-X-mental retardation-related protein 1) are RNA-binding proteins that have been demonstrated to impact miRNA-mediated, post-transcriptional gene regulation, and required for embryonic and postnatal development of muscle tissue. It can regulate intracellular transport and local translation of certain mRNAs. The FXR1 exists some isoforms with the MW 69 kDa and 60 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for FXR1 antibody 13194-1-AP | Download protocol |
| IHC protocol for FXR1 antibody 13194-1-AP | Download protocol |
| IP protocol for FXR1 antibody 13194-1-AP | Download protocol |
| WB protocol for FXR1 antibody 13194-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The FXR1 Polyclonal antibody (Cat# 13194-1-AP) has 35 citations spanning journals including Cell, Cancer Cell, Nature Communications, Neuron, Cell Reports, Molecular Cell, Science Advances, and eLife. Research fields covered include cancer biology (colorectal, glioma, lung, osteosarcoma), RNA biology and post-transcriptional regulation, neuroscience, metabolism, liver disease, virology, and developmental biology. Applications cited are predominantly WB, IF, IP, RIP, and IHC.
| Species | Application | Title |
|---|---|---|
Cancer Cell Integrative plasma and fecal metabolomics identify functional metabolites in adenoma-colorectal cancer progression and as early diagnostic biomarkers
| ||
Cell Competing Protein-RNA Interaction Networks Control Multiphase Intracellular Organization. | ||
Cell Metab Isoform usage as a distinct regulatory layer driving nutrient-responsive metabolic adaptation | ||
Nat Commun Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis | ||
Nat Commun Recessive mutations in muscle-specific isoforms of FXR1 cause congenital multi-minicore myopathy. | ||
Nat Commun Microtubule-based transport is essential to distribute RNA and nascent protein in skeletal muscle |
Reviews
What customers say
The single review for this product rates it 4 out of 5 stars. The reviewer used it on mouse heart tissue for Western blot and reported a strong band in the basal state, indicating clear and reliable detection under those conditions. Overall, the feedback is positive, highlighting the antibody's performance in WB applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Chao (Verified Customer) (01-30-2020) | Western blot show strong band in the basal state.
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