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FYCO1 Polyclonal antibody

FYCO1 Polyclonal Antibody for WB, ELISA

Cat No. 25730-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

FYCO1, RUFY3, ZFYVE7

Formulation:  PBS and Azide
PBS and Azide
Conjugate:  Unconjugated
Unconjugated
Size/Concentration: 

-/ -

Freight/Packing: -

Quantity

Please visit your regions distributor:


Tested Applications

Positive WB detected inHEK-293T cells, HeLa cells, LNCaP cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:2000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

25730-1-AP targets FYCO1 in WB, IF, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen FYCO1 fusion protein Ag22803 Predict reactive species
Full Name FYVE and coiled-coil domain containing 1
Calculated Molecular Weight 1478 aa, 167 kDa
Observed Molecular Weight167-180 kDa
GenBank Accession NumberBC007218
Gene Symbol FYCO1
Gene ID (NCBI) 79443
RRIDAB_3085821
Conjugate Unconjugated
FormLiquid
Purification MethodAntigen affinity purification
UNIPROT IDQ9BQS8
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FYCO1 is a Rab7 adapter protein that is implicated in the microtubule transport of autophagosomes. The encoded protein contains a RUN domain, a FYVE-type zinc finger domain, and Golgi dynamics (GOLD) domain. The encoded protein plays a role in microtubule plus end-directed transport of autophagic vesicles through interactions with the small GTPase Rab7, phosphatidylinositol-3-phosphate (PI3P), the autophagosome marker LC3, and the kinesin KIF5.

Protocols

Product Specific Protocols
WB protocol for FYCO1 antibody 25730-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

EMBO Rep

LRBA deficiency impairs autophagy and contributes to enhanced antigen presentation and T-cell dysregulation

Authors - Elena Sindram
IF, WB

Signal Transduct Target Ther

Nlp promotes autophagy through facilitating the interaction of Rab7 and FYCO1.

Authors - Wenchang Xiao
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