FYCO1 Polyclonal antibody

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  WB analysis using 25730-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 25730-1-AP (FYCO1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 25730-1-AP

  WB result of FYCO1 antibody (25730-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-FYCO1 transfected HeLa cells.

• Featured Product
• KD/KO Validated

FYCO1 Polyclonal Antibody for WB, ELISA

Cat No. 25730-1-AP

Publications(2)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF, ELISA

Synonyms

FYCO1, RUFY3, ZFYVE7

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Tested Applications

• Positive WB detected in: HEK-293T cells, HeLa cells, LNCaP cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IF: See 1 publications below

Product Information

25730-1-AP targets FYCO1 in WB, IF, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag22803

  Product name: Recombinant human FYCO1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-250 aa of BC007218

  Sequence: MNTKVTSDWYYARSPFLQPKLSSDIVGQLYELTEVQFDLASRGFDLDAAWPTFARRTLTTGSSAYLWKPPSRSSSMSSLVSSYLQTQEMVSNFDLNSPLNNEALEGFDEMRLELDQLEVREKQLQERMQQLDRENQELRAAVSQQGEQLQTERERGRTAAEDNVRLTCLVAELQKQWEVTQATQNTVKELQTCLQALELGAAEKEEDYHTALRRLESMLQPLAQELEATRDSLDKKNQHLASFPGWLAMA

• Full Name: FYVE and coiled-coil domain containing 1
• Calculated Molecular Weight: 1478 aa, 167 kDa
• Observed Molecular Weight: 167-180 kDa
• GenBank Accession Number: BC007218
• Gene Symbol: FYCO1
• Gene ID (NCBI): 79443
• RRID: AB_3085821
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9BQS8
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

FYCO1 is a Rab7 adapter protein that is implicated in the microtubule transport of autophagosomes. The encoded protein contains a RUN domain, a FYVE-type zinc finger domain, and Golgi dynamics (GOLD) domain. The encoded protein plays a role in microtubule plus end-directed transport of autophagic vesicles through interactions with the small GTPase Rab7, phosphatidylinositol-3-phosphate (PI3P), the autophagosome marker LC3, and the kinesin KIF5.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	EMBO Rep; LRBA deficiency impairs autophagy and contributes to enhanced antigen presentation and T-cell dysregulation; Authors - Elena Sindram
	IF, WB	Signal Transduct Target Ther; Nlp promotes autophagy through facilitating the interaction of Rab7 and FYCO1.; Authors - Wenchang Xiao