|Positive WB detected in||mouse kidney tissue, HeLa cells|
|Positive IHC detected in||human kidney tissue|
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
|Western Blot (WB)||WB : 1:500-1:1000|
|Immunohistochemistry (IHC)||IHC : 1:20-1:200|
|Sample-dependent, check data in validation data gallery|
18175-1-AP targets Frizzled 10 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.
|Tested Reactivity||human, mouse, rat|
|Cited Reactivity||chicken, goat, human, zebrafish|
|Host / Isotype||Rabbit / IgG|
|Immunogen||Frizzled 10 fusion protein Ag12867|
|Full Name||frizzled homolog 10 (Drosophila)|
|Calculated molecular weight||581 aa, 65 kDa|
|Observed molecular weight||65 kDa|
|GenBank accession number||BC074998|
|Gene ID (NCBI)||11211|
|Purification Method||Antigen affinity purification|
|Storage Buffer||PBS with 0.02% sodium azide and 50% glycerol pH 7.3.|
|Storage Conditions||Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.|
FZD10 (Frizzled homolog 10) is a member of the frizzled gene family, and considered as a seven-transmembrane Wnt-signaling receptor. FZD10 is expressed at high levels on the cell surface of almost all synovial sarcoma tissues but absent in most normal organs except for placenta. Up-regulation of FZD10 mRNA in several types of human cells might lead to carcinogenesis through activation of the beta-catenin-TCF signaling pathway with some class of WNTs.
Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3β Complex in Hypophosphatasia.
Integrative Analysis of Methylome and Transcriptome Reveals the Regulatory Mechanisms of Hair Follicle Morphogenesis in Cashmere Goat.
Coupling of apical-basal polarity and PCP to interpret the Wnt signaling gradient and orient feather branch.
Int J Oncol
Notch1 promotes hepatitis B virus X protein-induced hepatocarcinogenesis via Wnt/β-catenin pathway.
Non coding RNA analysis in fibrolamellar hepatocellular carcinoma.
Wnt/β-catenin signaling directly regulates Foxj1 expression and ciliogenesis in zebrafish Kupffer's vesicle.
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
Sylvain (Verified Customer) (12-11-2018)