Frizzled 10 Polyclonal antibody

Frizzled 10 Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat and More (3)

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 18175-1-AP

Synonyms

CD350, Frizzled 10, FZ 10, FZD10, FzE7, hFz10



Tested Applications

Positive WB detected inmouse kidney tissue, HeLa cells
Positive IHC detected inhuman kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

18175-1-AP targets Frizzled 10 in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity chicken, goat, human, zebrafish
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen Frizzled 10 fusion protein Ag12867
Full Name frizzled homolog 10 (Drosophila)
Calculated molecular weight 581 aa, 65 kDa
Observed molecular weight 65 kDa
GenBank accession numberBC074998
Gene symbol FZD10
Gene ID (NCBI) 11211
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

FZD10 (Frizzled homolog 10) is a member of the frizzled gene family, and considered as a seven-transmembrane Wnt-signaling receptor. FZD10 is expressed at high levels on the cell surface of almost all synovial sarcoma tissues but absent in most normal organs except for placenta. Up-regulation of FZD10 mRNA in several types of human cells might lead to carcinogenesis through activation of the beta-catenin-TCF signaling pathway with some class of WNTs.

Protocols

Product Specific Protocols
WB protocol for Frizzled 10 antibody 18175-1-APDownload protocol
IHC protocol for Frizzled 10 antibody 18175-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Theranostics

Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3β Complex in Hypophosphatasia.

Authors - Wenjia Liu
goatIHC

Cells

Integrative Analysis of Methylome and Transcriptome Reveals the Regulatory Mechanisms of Hair Follicle Morphogenesis in Cashmere Goat.

Authors - Shanhe Wang
chickenIHC,IF

Development

Coupling of apical-basal polarity and PCP to interpret the Wnt signaling gradient and orient feather branch.

Authors - Jianqiong Lin
humanWB

Int J Oncol

Notch1 promotes hepatitis B virus X protein-induced hepatocarcinogenesis via Wnt/β-catenin pathway.

Authors - Qian Sun
humanWB

Oncotarget

Non coding RNA analysis in fibrolamellar hepatocellular carcinoma.

Authors - Benjamin A Farber
zebrafishWB

Development

Wnt/β-catenin signaling directly regulates Foxj1 expression and ciliogenesis in zebrafish Kupffer's vesicle.

Authors - Alissa Caron

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Sylvain (Verified Customer) (12-11-2018)

Perfect signal!

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: human colon cancer