Tested Applications
| Positive WB detected in | mouse kidney tissue, HeLa cells |
| Positive IHC detected in | human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF-P detected in | human kidney tissue |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:20-1:200 |
| Immunofluorescence (IF)-P | IF-P : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
18175-1-AP targets Frizzled 10 in WB, IHC, IF-P, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, chicken, zebrafish, goat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag12867 Product name: Recombinant human FZD10 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 23-229 aa of BC074998 Sequence: SMDMERPGDGKCQPIEIPMCKDIGYNMTRMPNLMGHENQREAAIQLHEFAPLVEYGCHGHLRFFLCSLYAPMCTEQVSTPIPACRVMCEQARLKCSPIMEQFNFKWPDSLDCRKLPNKNDPNYLCMEAPNNGSDEPTRGSGLFPPLFRPQRPHSAQEHPLKDGGPGRGGCDNPGKFHHVEKSASCAPLCTPGVDVYWSREDKRFAVV Predict reactive species |
| Full Name | frizzled homolog 10 (Drosophila) |
| Calculated Molecular Weight | 581 aa, 65 kDa |
| Observed Molecular Weight | 65 kDa |
| GenBank Accession Number | BC074998 |
| Gene Symbol | Frizzled 10 |
| Gene ID (NCBI) | 11211 |
| RRID | AB_2108942 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9ULW2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
FZD10 (Frizzled homolog 10) is a member of the frizzled gene family, and considered as a seven-transmembrane Wnt-signaling receptor. FZD10 is expressed at high levels on the cell surface of almost all synovial sarcoma tissues but absent in most normal organs except for placenta. Up-regulation of FZD10 mRNA in several types of human cells might lead to carcinogenesis through activation of the beta-catenin-TCF signaling pathway with some class of WNTs.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for Frizzled 10 antibody 18175-1-AP | Download protocol |
| IHC protocol for Frizzled 10 antibody 18175-1-AP | Download protocol |
| WB protocol for Frizzled 10 antibody 18175-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Frizzled 10 (FZD10) Polyclonal antibody (Cat# 18175-1-AP) has 9 citations across journals including Nature Communications, Theranostics, mAbs, International Journal of Oncology, Cells, Oncotarget, Poultry Science, and Development. Research fields span Wnt/β-catenin signaling, nephrogenesis, stem cell lineage switching, therapeutic angiogenesis, hepatocarcinogenesis, hair follicle morphogenesis, hepatocellular carcinoma, feather development, and ciliogenesis.
| Species | Application | Title |
|---|---|---|
Nat Commun Progenitor translatome changes coordinated by Tsc1 increase perception of Wnt signals to end nephrogenesis. | ||
Theranostics Alkaline Phosphatase Controls Lineage Switching of Mesenchymal Stem Cells by Regulating the LRP6/GSK3β Complex in Hypophosphatasia. | ||
MAbs Coactivation of Tie2 and Wnt signaling using an antibody-R-spondin fusion potentiates therapeutic angiogenesis and vessel stabilization in hindlimb ischemia | ||
Int J Oncol Notch1 promotes hepatitis B virus X protein-induced hepatocarcinogenesis via Wnt/β-catenin pathway. | ||
Cells Integrative Analysis of Methylome and Transcriptome Reveals the Regulatory Mechanisms of Hair Follicle Morphogenesis in Cashmere Goat. | ||
Reviews
What customers say
Customer reviews for this antibody are mixed. One user reported excellent results in immunohistochemistry on human colon cancer tissue, describing a "perfect signal" at a 1:100 dilution. Another user found the immunofluorescence staining on fibroblasts (1:50 dilution) less impressive, noting it was not as good as related Frizzled 5, 7, or 9 antibodies from the same company. Overall, the antibody performs well for IHC but may show weaker staining in some IF applications.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Susanne (Verified Customer) (04-17-2023) | staining was not so nice like Frizzled 5, 7 or 9 from Proteintech
 |
Sylvain (Verified Customer) (12-11-2018) | Perfect signal!
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