Fas/CD95 Recombinant monoclonal antibody, PBS Only

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  IF Staining of Raji using 83400-5-RR (same clone as 83400-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using Fas/CD95 antibody (83400-5-RR, Clone: 240340E11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83400-5-PBS in a different storage buffer formulation.

• 

  IF Staining of Raji using 83400-5-RR (same clone as 83400-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using Fas/CD95 antibody (83400-5-RR, Clone: 240340E11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83400-5-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 83400-5-RR (same clone as 83400-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Fas/CD95 antibody (83400-5-RR, Clone: 240340E11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83400-5-PBS in a different storage buffer formulation.

• 

  IF Staining of Jurkat using 83400-5-RR (same clone as 83400-5-PBS)

  Immunofluorescent analysis of (4% PFA) fixed Jurkat cells using Fas/CD95 antibody (83400-5-RR, Clone: 240340E11 ) at dilution of 1:250 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 83400-5-PBS in a different storage buffer formulation.

• 

  Affinity and Kinetic Characterization of 83400-5-RR

  Biolayer interferometry (BLl) kinetic assays of 83400-5-RR against Human Fas/CD95 were performed. The affinity constant is 0.927 nM.

Fas/CD95 Uni-rAb^® Recombinant Antibody for IF/ICC, ELISA

Cat No. 83400-5-PBS

Clone No.240340E11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IF/ICC, ELISA

Synonyms

FAS, APT1, Apoptosis-mediating surface antigen FAS, Apo-1 antigen, APO-1

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Product Information

83400-5-PBS targets Fas/CD95 in IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Fusion Protein

• Full Name: Fas (TNF receptor superfamily, member 6)
• Calculated Molecular Weight: 38 kDa
• GenBank Accession Number: NM_000043.6
• Gene Symbol: Fas/CD95
• Gene ID (NCBI): 355
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P25445-1
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Fas (CD95/APO-1) is a transmembrane glycoprotein belonging to the tumor necrosis factor (TNF) receptor superfamily. It can mediate apoptosis by ligation with an agonistic anti-Fas antibody or Fas ligand. Stimulation of Fas results in the aggregation of its intracellular death domains, leading to the formation of the death-inducing signaling complex (DISC). FAS-mediated apoptosis may have a role in the induction of peripheral tolerance, in the antigen-stimulated suicide of mature T-cells, or both.