FceR1a Polyclonal antibody

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  IHC staining of mouse spleen using 32501-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32501-1-AP (FceR1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse spleen using 32501-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse spleen tissue slide using 32501-1-AP (FceR1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 32501-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 32501-1-AP (FceR1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse lung using 32501-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 32501-1-AP (FceR1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

FceR1a Polyclonal Antibody for IHC, ELISA

Cat No. 32501-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

mouse

Applications

IHC, ELISA

Synonyms

High affinity immunoglobulin epsilon receptor subunit alpha, Fcr 5, FcERI, FceR1, Fce1a

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Tested Applications

• Positive IHC detected in: mouse spleen tissue, mouse lung tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

32501-1-AP targets FceR1a in IHC, ELISA applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Recombinant protein

• Full Name: Fc receptor, IgE, high affinity I, alpha polypeptide
• Calculated Molecular Weight: 29 kDa
• GenBank Accession Number: NM_010184.2
• Gene Symbol: FceR1a
• Gene ID (NCBI): 14125
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity Purification
• UNIPROT ID: P20489
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Fc epsilon Receptor I alpha (FceR1a) is a single-pass type I membrane protein that contains 2 immunoglobulin-like domains. FceR1a forms a tetrameric complex, FceRI, with one beta and two gamma subunits (PMID: 19406478). FceRI is the high-affinity receptor for the Fc region of immunoglobulin E (IgE) (PMID: 1532373). It is expressed on mast cells and basophils and plays a central role in the allergic response (PMID: 2148316).