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Ferritin heavy chain Polyclonal antibody, PBS Only (11682-1-PBS)

Filter: AllWBIPIHCIF/ICC

WB analysis using 11682-1-AP (same clone as 11682-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.
WB analysis of HeLa using 11682-1-AP (same clone as 11682-1-PBS)
WB result of Ferritin heavy chain antibody (11682-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Ferritin heavy chain transfected HeLa cells. This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.
WB analysis using 11682-1-AP (same clone as 11682-1-PBS)
Various lysates were subjected to SDS PAGE followed by western blot with 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.
IP experiment of HeLa using 11682-1-AP (same clone as 11682-1-PBS)
IP result of anti-Ferritin heavy chain (IP:11682-1-AP, 4ug; Detection:11682-1-AP 1:3000) with HeLa cells lysate 1600 ug. This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.
IHC staining of rat liver using 11682-1-AP (same clone as 11682-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 11682-1-AP (same clone as 11682-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.
IHC staining of rat liver using 11682-1-AP (same clone as 11682-1-PBS)
Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.
IHC staining of human liver using 11682-1-AP (same clone as 11682-1-PBS)
Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 11682-1-AP (same clone as 11682-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.
IHC staining of mouse liver using 11682-1-AP (same clone as 11682-1-PBS)
Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 11682-1-AP (Ferritin heavy chain antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 11682-1-AP (same clone as 11682-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ferritin heavy chain antibody (11682-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.
IF Staining of HeLa using 11682-1-AP (same clone as 11682-1-PBS)
Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using Ferritin heavy chain antibody (11682-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 11682-1-PBS in a different storage buffer formulation.

Featured Product
KD/KO Validated

Ferritin heavy chain Polyclonal antibody, PBS Only

Name: Ferritin heavy chain Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 11682-1-PBS

Ferritin heavy chain Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Cat No. 11682-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Synonyms

Ferritin, FTH1, Cell proliferation-inducing gene 15 protein, EC:1.16.3.1, Ferritin heavy chain, N-terminally processed

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

11682-1-PBS targets Ferritin heavy chain in WB, IHC, IF/ICC, IP, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	CatNo: Ag2290 Product name: Recombinant human FTH1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-183 aa of BC000857 Sequence: MTTASTSQVRQNYHQDSEAAINRQINLELYASYVYLSMSYYFDRDDVALKNFAKYFLHQSHEEREHAEKLMKLQNQRGGRIFLQDIKKPDCDDWESGLNAMECALHLEKNVNQSLLELHKLATDKNDPHLCDFIETHYLNEQVKAIKELGDHVTNLRKMGAPESGLAEYLFDKHTLGDSDNES Predict reactive species
Full Name	ferritin, heavy polypeptide 1
Calculated Molecular Weight	183 aa, 21 kDa
Observed Molecular Weight	21 kDa
GenBank Accession Number	BC000857
Gene Symbol	FTH1
Gene ID (NCBI)	2495
RRID	AB_3669131
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	P02794
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

Ferritin heavy chain(FTH1, ferritin, heavy polypeptide 1 ), as a 21 kDa subunit of the ferritin complex, exhibits ferroxidase activity, which serves an essential role in catalyzing the conversion of the ferrous ions (Fe2+) to the ferric form (Fe3+). FTH1 regulates angiogenesis during inflammation and malignancy by binding to high molecular weight kininogen (HKa) and blocking its anti-angiogenic activity on endothelial cells in vitro and in vivo(PMID: 33260500). FTH1 has been shown to protect proximal tube epithelial cells and kidneys against the activity of free iron in reactive oxygen species generation. FTH1 plays an important role in the maintenance of the cellular iron balance in ferroptosis.