Rat Ferritin light chain 1 Recombinant monoclonal antibody, PBS Only

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  WB analysis of mouse testis using 84731-7-RR (same clone as 84731-7-PBS)

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 84731-7-RR (Ferritin light chain 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84731-7-PBS in a different storage buffer formulation.

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  WB analysis using 84731-7-RR (same clone as 84731-7-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84731-7-RR (Ferritin light chain 1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84731-7-PBS in a different storage buffer formulation.

• 

  WB analysis of rat liver using 84731-7-RR (same clone as 84731-7-PBS)

  rat liver tissue were subjected to SDS PAGE followed by western blot with 84731-7-RR (Ferritin light chain 1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84731-7-PBS in a different storage buffer formulation.

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  IHC staining of mouse liver using 84731-7-RR (same clone as 84731-7-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse liver tissue slide using 84731-7-RR (Ferritin light chain 1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84731-7-PBS in a different storage buffer formulation.

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  IF Staining of mouse liver using 84731-7-RR (same clone as 84731-7-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded mouse liver tissue using Ferritin light chain 1 antibody (84731-7-RR, Clone: 242299F7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84731-7-PBS in a different storage buffer formulation.

Ferritin light chain 1 Uni-rAb^® Recombinant Antibody for WB, IHC, IF-P, Indirect ELISA

Cat No. 84731-7-PBS

Clone No.242299F7

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, IF-P, Indirect ELISA

Synonyms

242299F7, Ferritin L subunit 1, Ftl, Ftl1

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Product Information

84731-7-PBS targets Ferritin light chain 1 in WB, IHC, IF-P, Indirect ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg2434

  Product name: Recombinant Rat Ferritin light chain 1 protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 1-183 aa of NM_022500.4

  Sequence: MTSQIRQNYSTEVEAAVNRLVNLHLRASYTYLSLGFFFDRDDVALEGVGHFFRELAEEKREGAERLLKLQNERGGRALFQDVQKPSQDEWGKTLEAMEAALALEKNLNQALLDLHALGSARTDPHLCDFLESHFLDKEVKLIKKMGNHLTNLRRVAGPQPAQTGVAQASLGEYLFERLTLKHD

• Full Name: Ferritin light chain 1
• Calculated Molecular Weight: 21 kDa
• Observed Molecular Weight: 18-20 kDa
• GenBank Accession Number: NM_022500.4
• Gene Symbol: Ftl
• Gene ID (NCBI): 29292
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P02793
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

FTL belongs to the ferritin family. It stores iron in a soluble, non-toxic, readily available form. FTL is important for iron homeostasis. It plays a role in delivery of iron to cells. FTL mediates iron uptake in capsule cells of the developing kidney. Mutation of FTL will cause hereditary hyperferritinemia-cataract syndrome (HHCS) or neurodegeneration with brain iron accumulation type 3 (NBIA3). Ferritin light polypeptide (FTL) and ferritin heavy polypeptide (FTH1) were the main constituents the striatum and cerebellar cortex revealed.