Fhl1 Polyclonal antibody

Fhl1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Cat no : 51015-1-AP

Synonyms

Fhl1, four and a half LIM domains 1, KyoT

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Validation Data Gallery

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  WB analysis of human heart using 51015-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 51015-1-AP (Fhl1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human ovary tumor using 51015-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 51015-1-AP (Fhl1 antibody) at dilution of 1:50 (under 10x lens).

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Tested Applications

• Positive WB detected in: human heart tissue
• Positive IHC detected in: human ovary tumor tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

51015-1-AP targets Fhl1 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: four and a half LIM domains 1
• Calculated Molecular Weight: 32 kDa
• Observed Molecular Weight: 32 kDa
• GenBank Accession Number: BC031120
• Gene Symbol: Fhl1
• Gene ID (NCBI): 14199
• RRID: AB_615005
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.